Invitrogen

CCL3 Recombinant Rabbit Monoclonal Antibody (013)

View all (37) CCL3 (MIP-1 alpha) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-31275

Applications
Tested Dilution
Publications

ELISA (ELISA)

1:5,000-1:10,000
-
Product Specifications

Species Reactivity

Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

013

Immunogen

Recombinant Rat CCL3/Mip1a Protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2786914

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Rat CCL3/Mip1a.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; RP23-320E6.7; small inducible cytokine A3; Small-inducible cytokine A3

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Gene Aliases: Ccl3; MIP-1a; Mip1a; Scya3

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UniProt ID: (Rat) P50229

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Entrez Gene ID: (Rat) 25542

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Function(s)
protein kinase activity calcium-dependent protein kinase C activity chemokine activity heparin binding phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding
Process(es)
MAPK cascade osteoblast differentiation cell activation monocyte chemotaxis protein phosphorylation calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response cytoskeleton organization positive regulation of cytosolic calcium ion concentration cell-cell signaling regulation of cell shape response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration negative regulation of bone mineralization neutrophil chemotaxis leukocyte chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production response to drug positive regulation of catalytic activity eosinophil degranulation protein kinase B signaling positive regulation of neuron apoptotic process astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport regulation of sensory perception of pain cell chemotaxis positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to organic cyclic compound granulocyte chemotaxis positive regulation of natural killer cell chemotaxis
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