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Invitrogen
CCL3 (MIP-1 alpha) Monoclonal Antibody (DNT3CC), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 2
CCL3 (MIP-1 alpha) Antibody (46-7532-82) in Flow
BALB/c thioglycolate-elicited peritoneal macrophages were unstimulated (left) or stimulated for 5 hours with LPS (right) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03). Cells were intracellularly stained with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.25 µg of Anti-Mouse CCL3 (MIP-1 alpha) PerCP-eFluor® 710, using the Intracellular Fixation & Permeabilization Buffer S... View More
Product Details
46-7532-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DNT3CC
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573842
Product Specific Information
Description: This DNT3CC monoclonal antibody reacts with mouse CCL3. CCL3, also known as MIP-1 alpha (Macrophage Inflammatory Protein 1 alpha), is a member of the CC- subfamily of chemokines and is most closely related to CCL4, or MIP-1 beta. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 will attract monocytes, macrophages, and dendritic cells, CCL3 preferentially attracts CD8+ T cells, while CD4+ T cells are more responsive to CCL4. In addition to its chemotactic functions, CCL3 induces inflammatory cytokine secretion, mast cell degranulation, and NK cell activation. It has also been reported to inhibit hematopoetic stem cell proliferation and may be responsible for the maintenance of these cells in a quiescent state. CCL3 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL4 and CCL5 (RANTES).
Applications Reported: This DNT3CC antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This DNT3CC antibody has been tested intracellular staining of stimulated mouse spleen cells followed by flow cytometric analysis using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; Heparin-binding chemotaxis protein; L2G25B; LD78-alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1 alpha; MIP-1-alpha; MIP1 (a); mip1 alpha; RP23-320E6.7; SIS-alpha; small inducible cytokine A3; Small-inducible cytokine A3; TY-5
Gene Aliases: AI323804; Ccl3; G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3
UniProt ID: (Mouse) P10855
Entrez Gene ID: (Mouse) 20302
chemotaxis
inflammatory response
immune response
positive regulation of cytosolic calcium ion concentration
regulation of cell shape
neutrophil chemotaxis
leukocyte chemotaxis
positive regulation of tumor necrosis factor production
positive regulation of neuron apoptotic process
astrocyte cell migration
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
macrophage chemotaxis
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
cell chemotaxis
Performance Guarantee
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