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CCL4 Antibody (500-P213-1MG) in WB
Western Blot: To detect Murine MIP-1beta (CCL4) by Western Blot analysis CCL4 Polyclonal Antibody (Product # 500-P213-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1beta (CCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P213-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MIP-1beta (CCL4)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929844
Product Specific Information
AA Sequence of recombinant protein: APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1beta (CCL4). Anti-Murine MIP-1beta (CCL4)-specific antibody was purified by affinity chromatography employing an immobilized Murine MIP-1beta (CCL4) matrix.
Sandwich ELISA: To detect Murine MIP-1beta (CCL4) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine MIP-1beta (CCL4) (500-P213Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1beta (CCL4).
Western Blot: To detect Murine MIP-1beta (CCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1beta (CCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACT-2; ACT2; C-C motif chemokine 4; Immune activation protein 2; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; MIP-1-beta; MIP1-beta; MIP1beta; Protein H400; RP23-320E6.8; SIS-gamma; small inducible cytokine A4; Small-inducible cytokine A4
Gene Aliases: Act-2; AT744.1; Ccl4; MIP-1B; Mip1b; Scya4
UniProt ID: (Mouse) P14097
Entrez Gene ID: (Mouse) 20303
chemotaxis
inflammatory response
immune response
response to toxic substance
leukocyte chemotaxis
positive regulation of tumor necrosis factor production
cellular response to drug
positive regulation of vascular permeability
negative regulation by host of viral transcription
positive regulation of calcium-mediated signaling
positive regulation of calcium ion transport
positive regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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