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CCL4 Antibody (PA5-47785) in WB
Western blot analysis of CCL4 in canine peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 5 µg/mL PMA and 500 ng/mL Ionomycin overnight. Samples were incubated in CCL4 polyclonal antibody (Product # PA5-47785) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for CCL4/MIP‚1β at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant canine CCL4/MIP-1 beta Ala24-Asn92
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607322
Product Specific Information
In direct ELISAs, approximately 10% cross-reactivity with recombinant human CCL4/MIP-1 beta and recombinant mouse CCL4/MIP-1 beta is observed and less than 1% cross-reactivity with recombinant viral CCL4/MIP-1 beta is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 4; CC chemokine ligand 4; MGC104418; MGC126025; MGC126026; MIP1-beta; MIP1beta; RP23-320E6.8; Small-inducible cytokine A4
Gene Aliases: CCL4; SCYA4
UniProt ID: (Dog) Q68AZ0
Entrez Gene ID: (Dog) 448786
monocyte chemotaxis
inflammatory response
G-protein coupled receptor signaling pathway
response to toxic substance
neutrophil chemotaxis
cellular response to drug
positive regulation of GTPase activity
negative regulation by host of viral transcription
lymphocyte chemotaxis
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of calcium ion transport
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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