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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CCL5 (RANTES) Antibodies
          Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Invitrogen

          CCL5 (RANTES) Recombinant Superclonal™ Antibody (25HCLC)

          2 Published Figures
          7 References
          View all (33) CCL5 (RANTES) antibodies

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          Datasheet
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          Cite CCL5 (RANTES) Recombinant Superclonal™ Antibody (25HCLC)

          • Antibody Testing Data (3)
          • Published Figures (2)
          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CCL5 (RANTES) Antibody (710001) in ICC/IF

          Immunofluorescence analysis of CCL5/RANTES was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CCL5/RANTES (25HCLC) Recombinant Rabbit Superclonal™ Antibody (Product # 710001) at 2 µg/mL in 0.1% BSA and ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CCL5 (RANTES) Antibody in Western Blot (WB)
          CCL5 (RANTES) Antibody in Immunohistochemistry (IHC)
          CCL5 (RANTES) Antibody in Immunohistochemistry (IHC)

          Product Details

          710001

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1 µg/mL
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Superclonal

          Type

          Antibody

          Clone

          25HCLC

          Immunogen

          proprietary

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2532515

          Product Specific Information

          This antibody is predicted to react with rat and human based on sequence homology.

          Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Target Information

          CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

          View more View less

          Gene Aliases: CCL5; D17S136E; eoCP; MuRantes; RANTES; SCYA5; SIS-delta; SISd; TCP228

          View more View less

          UniProt ID: (Human) P13501, (Mouse) P30882

          View more View less

          Entrez Gene ID: (Human) 6352, (Rat) 81780, (Mouse) 20304

          View more View less

          Function(s)
          phosphatidylinositol phospholipase C activity protein kinase activity protein binding chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine activity
          Process(es)
          MAPK cascade chronic inflammatory response protein phosphorylation calcium ion transport cellular calcium ion homeostasis exocytosis inflammatory response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling aging response to virus response to bacterium response to toxic substance response to salt stress positive regulation of gene expression positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling response to organic cyclic compound response to activity positive regulation of smooth muscle cell migration dibenzo-p-dioxin metabolic process positive regulation of cell migration pseudopodium assembly positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to nutrient levels response to lipopolysaccharide response to insulin positive regulation of cell-cell adhesion mediated by integrin response to cytokine positive regulation of homotypic cell-cell adhesion response to interferon-gamma response to tumor necrosis factor T-helper 1 cell diapedesis positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling cellular protein complex assembly response to estrogen negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus negative regulation of viral genome replication positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion eosinophil chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization response to glucocorticoid positive regulation of calcium ion transport negative regulation of cell death positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process response to cholesterol cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to vitamin K cellular response to morphine cellular response to cytokine stimulus cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to ethanol cellular response to lipid cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to alkyl hydroperoxide cellular response to transforming growth factor beta stimulus positive regulation of monocyte chemotaxis regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis dendritic cell chemotaxis monocyte chemotaxis regulation of chronic inflammatory response chemotaxis positive regulation of activation of JAK2 kinase activity neutrophil chemotaxis positive regulation of GTPase activity positive regulation of viral genome replication positive regulation of innate immune response positive regulation of translational initiation positive regulation of JAK-STAT cascade macrophage chemotaxis positive regulation of inflammatory response positive regulation of ERK1 and ERK2 cascade positive regulation of defense response to virus by host immune response extracellular negative regulation of signal transduction negative regulation of receptor activity positive regulation of chemokine (C-X-C motif) ligand 2 production
          It has to be done as per old AB suggested Products section.
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