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          • Primary Antibodies ›
          • CCL5 (RANTES) Antibodies

          Invitrogen

          CCL5 (RANTES) Recombinant Rabbit Monoclonal Antibody (JM03-45)

          View all (33) CCL5 (RANTES) antibodies

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          Datasheet
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          Cite CCL5 (RANTES) Recombinant Rabbit Monoclonal Antibody (JM03-45)

          • Antibody Testing Data (5)
          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CCL5 (RANTES) Antibody (MA5-45008) in ICC/IF

          Immunocytochemistry analysis of RANTES in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in RANTES Monoclonal antibody (Product # MA5-45008) using a dilution of 1:50 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®48... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          CCL5 (RANTES) Antibody in Immunocytochemistry (ICC/IF)
          CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CCL5 (RANTES) Antibody in Western Blot (WB)

          Product Details

          MA5-45008

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          JM03-45

          Immunogen

          Recombinant protein within human RANTES aa 1-91.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 0.05% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931462

          Target Information

          CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

          View more View less

          Gene Aliases: CCL5; D17S136E; eoCP; MuRantes; RANTES; SCYA5; SIS-delta; SISd; TCP228

          View more View less

          UniProt ID: (Human) P13501, (Mouse) P30882

          View more View less

          Entrez Gene ID: (Human) 6352, (Mouse) 20304

          View more View less

          Function(s)
          phosphatidylinositol phospholipase C activity protein kinase activity protein binding chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine activity
          Process(es)
          MAPK cascade dendritic cell chemotaxis chronic inflammatory response monocyte chemotaxis regulation of chronic inflammatory response calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling aging response to virus response to toxic substance response to salt stress positive regulation of activation of JAK2 kinase activity positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling response to activity positive regulation of smooth muscle cell migration dibenzo-p-dioxin metabolic process positive regulation of cell migration neutrophil chemotaxis positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to insulin positive regulation of cell-cell adhesion mediated by integrin positive regulation of homotypic cell-cell adhesion positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling positive regulation of GTPase activity cellular protein complex assembly response to estrogen negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus positive regulation of viral genome replication negative regulation of viral genome replication positive regulation of innate immune response positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of translational initiation positive regulation of JAK-STAT cascade eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of inflammatory response regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization response to glucocorticoid positive regulation of calcium ion transport positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to amino acid stimulus cellular response to vitamin K cellular response to morphine cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to ethanol cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to alkyl hydroperoxide cellular response to transforming growth factor beta stimulus positive regulation of monocyte chemotaxis regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis positive regulation of defense response to virus by host immune response positive regulation of gene expression pseudopodium assembly response to cytokine response to interferon-gamma response to tumor necrosis factor T-helper 1 cell diapedesis negative regulation of cell death extracellular negative regulation of signal transduction negative regulation of receptor activity positive regulation of chemokine (C-X-C motif) ligand 2 production
          It has to be done as per old AB suggested Products section.
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