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Invitrogen
CCL7 (MCP-3) Monoclonal Antibody (OLGMASCE), eFluor™ 660, eBioscience™
FIGURE: 1 / 1
CCL7 (MCP-3) Antibody (50-7077-41) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated 5 hours with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were intracellularly stained with Anti-Human CD14 PE (Product # 12-0149-42) and Anti-Human CCL7 eFluor® 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.
Product Details
50-7077-41
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OLGMASCE
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574275
Product Specific Information
Description: This OLGMASCE monoclonal antibody reacts with human CCL7. CCL7, also known as MCP-3 (Monocyte Chemotactic Protein 3), is a member of the CC- subfamily of chemokines and is most closely related to CCL2 (MCP-1) and CCL8 (MCP-2). They are secreted by a variety of cell types in response to inflammatory stimuli and play critical roles in the recruitment of leukocytes to areas of inflammation. While all three MCP proteins are potent chemoattractants of monocytes and T cells, CCL7 appears to have the broadest range of activity, as it has also been demonstrated to attract activated NK cells, eosinophils, basophils, and neutrophils. CCL7 signals via the g protein-coupled receptors CCR1 and CCR2, both of which are shared with other CC-chemokines.
Applications Reported: This OLGMASCE antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This OLGMASCE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 7; chemokine (C-C motif) ligand 7; H-MCP-3; MCP-3; MGC138463; MGC138465; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; small inducible cytokine A7 (monocyte chemotactic protein 3); Small-inducible cytokine A7
Gene Aliases: CCL7; FIC; MARC; MCP-3; MCP3; NC28; SCYA6; SCYA7
UniProt ID: (Human) P80098
Entrez Gene ID: (Human) 6354
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
response to gamma radiation
positive regulation of cell migration
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to ethanol
positive regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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