Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Abnova
CCNB1 Monoclonal Antibody (1C8)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
H00000891-M01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1C8
Immunogen
CCNB1 (NP_114172, 1 a.a. approximately 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Cell lysate).
Immunogen sequence: MALRVTRNSK INAENKAKIN MAGAKRVPTA PAATSKPGLR PRTALGDIGN KVSEQLQAKM PMKKEAKPSA TGKVIDKKLP KPLEKVPMLV
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023
Gene Aliases: CCNB; CCNB1
UniProt ID: (Human) P14635
Entrez Gene ID: (Human) 891
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
negative regulation of protein phosphorylation
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic spindle organization
mitotic nuclear envelope disassembly
mitotic metaphase plate congression
spermatogenesis
response to mechanical stimulus
negative regulation of gene expression
anaphase-promoting complex-dependent catabolic process
positive regulation of mRNA 3'-end processing
positive regulation of histone phosphorylation
tissue regeneration
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of cell cycle
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to organic cyclic compound
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
histone H3-S10 phosphorylation involved in chromosome condensation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support