Invitrogen

CCR2 Monoclonal Antibody (8C4)

View all (32) CCR2 (CD192) antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-49147

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

8C4

Immunogen

E.coli-derived human CCR2 recombinant protein (Position: M1-F125).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074805

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human THP-1 whole cell, human K562 whole cell, human Jurkat whole cell. IHC: human liver cancer tissue, human tonsil tissue. ICC/IF: HepG2 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

CCR2, a chemokine receptor, is a regulator of monocyte/macrophage traffickings and is upregulated in response to inflammation. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. The ligand for CCR2 is monocyte chemoattractant protein (MCP-1), a chemokine produced by endothelial cells, smooth muscle cells, and macrophages. Activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis and other inflammatory disorders including rheumatoid arthritis, alveolitis, and tumor infiltration. Two isoforms are produced by alternative splicing. CCR2 has been reported to be expressed in a wide variety of tissues, including blood, brain, heart, kidney, liver, lung, ovary, pancreas, spinal cord, spleen, thymus, and vessel. Increased expression of CCR2 has been documented in CD3+ lymphocytes in rheumatoid arthritis. ESTs have been isolated from liver/spleen and prostate libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C chemokine receptor type 2; C-C CKR-2; Cc-ckr2; CD192; CD192 antigen; chemokine (C-C motif) receptor 2; hCG_14621; MCP-1 receptor; MCP-1-R; Mcp1 receptor; Mcp1r; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Gene Aliases: CC-CKR-2; CCR-2; CCR2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R

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UniProt ID: (Human) P41597

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Entrez Gene ID: (Human) 729230

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Function(s)
chemokine receptor activity C-C chemokine receptor activity protein kinase binding CCR2 chemokine receptor binding protein homodimerization activity chemokine (C-C motif) ligand 5 binding
Process(es)
blood vessel remodeling dendritic cell chemotaxis inflammatory response to antigenic stimulus positive regulation of T-helper 1 type immune response negative regulation of type 2 immune response cellular calcium ion homeostasis chemotaxis inflammatory response immune response cellular defense response G-protein coupled receptor signaling pathway negative regulation of adenylate cyclase activity positive regulation of cytosolic calcium ion concentration JAK-STAT cascade aging response to wounding response to ionizing radiation regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of T cell chemotaxis negative regulation of angiogenesis cytokine-mediated signaling pathway cellular homeostasis negative regulation of cell migration negative regulation of axon extension forebrain development positive regulation of fever generation response to lipopolysaccharide positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production T-helper 17 cell chemotaxis positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative regulation of eosinophil degranulation fat cell differentiation positive regulation of neuron differentiation positive regulation of alpha-beta T cell proliferation positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of T cell activation positive regulation of apoptotic process by virus chemokine-mediated signaling pathway neuron death positive regulation of monocyte chemotaxis positive regulation of cell migration involved in sprouting angiogenesis positive regulation of immune complex clearance by monocytes and macrophages negative regulation of neural precursor cell proliferation positive regulation of monocyte extravasation positive regulation of CD8-positive, alpha-beta T cell extravasation positive regulation of astrocyte chemotaxis positive regulation of hematopoietic stem cell migration
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