Invitrogen

CCR6 Polyclonal Antibody

CCR6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CCR6 Antibody (PA5-29015) in IHC (P)

Immunohistochemical analysis of paraffin-embedded DU-145 xenograft, using CCR6 (Product # PA5-29015) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

CCR6 Antibody in Immunohistochemistry (IHC)

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CCR6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of CCR6 Polyclonal Antibody (Product # PA5-29015) was observed in HCT 116, HT-29, SW480, Jurkat, Raji, MOLT-4 and HeLa but not in Caco-2. {RE}

Product Details

PA5-29015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CCR6. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546491

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of CCL20 and Its Corresponding Receptor CCR6 Is Enhanced in Active Inflammatory Bowel Disease, and TLR3 Mediates CCL20 Expression in Colonic Epithelial Cells.

PloS one

Skovdahl HK,Granlund Av,Østvik AE,Bruland T,Bakke I,Torp SH,Damås JK,Sandvik AK

PA5-29015 was used in immunohistochemistry - paraffin section to assess the role of TLR3 in enterocytic CCL20 signaling

Tue Jun 14 00:00:00 UTC 2016

Expression of CCL20 and Its Corresponding Receptor CCR6 Is Enhanced in Active Inflammatory Bowel Disease, and TLR3 Mediates CCL20 Expression in Colonic Epithelial Cells.

PloS one

Skovdahl HK,Granlund Av,Østvik AE,Bruland T,Bakke I,Torp SH,Damås JK,Sandvik AK

PA5-29015 was used in immunohistochemistry - paraffin section to assess the role of TLR3 in enterocytic CCL20 signaling

Tue Jun 14 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22

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Gene Aliases: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22

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UniProt ID: (Human) P51684

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Entrez Gene ID: (Human) 1235

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Function(s)

receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity C-C chemokine binding

Process(es)

dendritic cell chemotaxis leukocyte migration involved in inflammatory response movement of cell or subcellular component chemotaxis immune response humoral immune response cellular defense response signal transduction G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of epithelial cell migration calcium-mediated signaling isotype switching to IgA isotypes cell chemotaxis positive regulation of sperm motility involved in capacitation chemokine-mediated signaling pathway lymphocyte migration T cell migration thymocyte migration DN2 thymocyte differentiation DN3 thymocyte differentiation regulation of T cell migration positive regulation of dendritic cell chemotaxis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCR6 Polyclonal Antibody

CCR6 Antibody (PA5-29015) in IHC (P)

Product Details

PA5-29015

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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