Invitrogen

CCR7 Monoclonal Antibody (4B12)

1 Published Figure
2 References
View all (69) CCR7 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-163

Applications
Tested Dilution
Publications

Western Blot (WB)

1:250-1:1,000
View 1 publication 1 publication

Flow Cytometry (Flow)

5 µg/1X10^6 cells
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

4B12

Immunogen

Rat basophilic leukaemia (RBL)-2H3 transfectants expressing mouse CCR7
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536868

Product Specific Information

MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes.

MA1-163 has been validated for Western blot on Jurkat cell lysates.

Target Information

CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; chemokine (C-C) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; Ebi1h; EVI1

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UniProt ID: (Human) P32248, (Mouse) P47774

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Entrez Gene ID: (Human) 1236, (Mouse) 12775

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Function(s)
G-protein coupled receptor activity C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity signal transducer activity chemokine receptor activity protein binding
Process(es)
establishment of T cell polarity positive regulation of cell-matrix adhesion dendritic cell chemotaxis myeloid dendritic cell chemotaxis positive regulation of dendritic cell antigen processing and presentation positive regulation of hypersensitivity positive regulation of humoral immune response inflammatory response immune response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of actin filament polymerization positive regulation of pseudopodium assembly ruffle organization response to lipopolysaccharide regulation of interferon-gamma production positive regulation of interleukin-12 production response to prostaglandin E chemokine (C-C motif) ligand 19 signaling pathway chemokine (C-C motif) ligand 21 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of phosphatidylinositol 3-kinase activity negative thymic T cell selection positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of JNK cascade homeostasis of number of cells regulation of interleukin-1 beta secretion positive regulation of T cell receptor signaling pathway release of sequestered calcium ion into cytosol positive regulation of filopodium assembly positive regulation of protein kinase B signaling positive regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus response to nitric oxide interleukin-12 secretion positive regulation of neutrophil chemotaxis activation of GTPase activity lymphocyte migration into lymph node mature conventional dendritic cell differentiation negative regulation of leukocyte apoptotic process positive regulation of cell motility positive regulation of dendritic cell chemotaxis positive regulation of immunological synapse formation positive regulation of T cell costimulation positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation regulation of dendritic cell dendrite assembly lymphocyte chemotaxis across high endothelial venule regulation of tolerance induction to self antigen chemotaxis signal transduction activation of JUN kinase activity positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis actin cytoskeleton organization positive regulation of tumor necrosis factor production positive regulation of T cell proliferation positive regulation of GTPase activity positive regulation of T-helper 1 cell differentiation positive regulation of receptor-mediated endocytosis lymph node development positive regulation of interleukin-1 beta secretion positive regulation of phagocytosis chemokine-mediated signaling pathway positive regulation of thymocyte migration
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