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CCRL2 Antibody (PA5-103189) in ICC/IF
Immunocytochemistry analysis of CCRL2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CCRL2 polyclonal antibody (Product # PA5-103189) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CCRL2(Accession O00421), corresponding to amino acid residues C147-K197.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852557
Product Specific Information
Antibody detects endogenous levels of total CCRL2.
Target Information
CCRL2 (C-C chemokine receptor-like 2), also known as chemokine receptor X, human chemokine receptor (HCR), CRAM-A or CRAM-B, is a seven pass transmembrane protein expressed by monocytes, neutrophils, and dendritic cells of immunal tissues (predominanly spleen, fetal liver, bone marrow and lymph node). It shares over 40% homology with other C-C chemokine receptors but does not share the conserved DRY motif which is among the important motifs necessary for signalling and ligand-binding. C-C chemokine receptors are G-protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: atypical chemokine receptor 5; C-C chemokine receptor-like 2; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; MGC116710; Putative MCP-1 chemokine receptor
Gene Aliases: ACKR5; CCR11; CCR6; CCRL2; CKRX; CRAM; CRAM-A; CRAM-B; HCR
UniProt ID: (Human) O00421
Entrez Gene ID: (Human) 9034, (Rat) 316019
Performance Guarantee
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