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Invitrogen
CCRL2 Chimeric Recombinant Rabbit Monoclonal Antibody (BZ5B8)
FIGURE: 1 / 3
CCRL2 Chimeric Antibody (MA5-48141) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CCRL2 in RAW264.7 cells using CCRL2 Chimeric Monoclonal Antibody (Product # MA5-48141). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CCRL2 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from lef... View More
Product Details
MA5-48141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BZ5B8
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide (aa 1-24) /KLH conjugate.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074584
Product Specific Information
Specificity: This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.
Preparation: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Target Information
CCRL2 (C-C chemokine receptor-like 2), also known as chemokine receptor X, human chemokine receptor (HCR), CRAM-A or CRAM-B, is a seven pass transmembrane protein expressed by monocytes, neutrophils, and dendritic cells of immunal tissues (predominanly spleen, fetal liver, bone marrow and lymph node). It shares over 40% homology with other C-C chemokine receptors but does not share the conserved DRY motif which is among the important motifs necessary for signalling and ligand-binding. C-C chemokine receptors are G-protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor-like 2; Chemokine receptor CCR11; G-protein coupled beta chemokine receptor; L-CCR; Lipopolysaccharide-inducible C-C chemokine receptor; MGC116710
Gene Aliases: 1810047I05Rik; Ackr5; CCR11; Ccrl2; Cmkbr1l2; E01; L-CCR; Lccr
UniProt ID: (Mouse) Q3UKM6
Entrez Gene ID: (Mouse) 54199
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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