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Invitrogen
CCT3 Monoclonal Antibody (12H4)
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FIGURE: 1 / 5
CCT3 Antibody (MA5-27872) in ICC/IF
Immunocytochemistry analysis of CCT3 using anti-CCT3 antibody (Product # MA5-27872). CCT3 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL mouse anti-CCT3 antibody (Product # MA5-27872) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualiz... View More
Product Details
MA5-27872
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
12H4
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CCT3 (497-536aa EPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744928
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control|WB: human Hela whole cell, human MCF-7 whole cell, human COLO-320 whole cell, human HepG2 whole cell. ICC/IF: A431 cell.
Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human COLO-320 whole cell, human HepG2 whole cell. ICC/IF: A431 cell.
Target Information
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCT-gamma; Chaperonin containing T-complex polypeptide 1 subunit 3; chaperonin containing TCP1, subunit 3 (gamma); hTRiC5; OTTHUMP00000025735; T-complex protein 1 subunit gamma; T-complex protein 1, gamma subunit; TCP-1-gamma; TCP1 (t-complex-1) ring complex, polypeptide 5
Gene Aliases: CCT-gamma; CCT3; CCTG; PIG48; TCP-1-gamma; TRIC5
UniProt ID: (Human) P49368
Entrez Gene ID: (Human) 7203
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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