Invitrogen

CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), Biotin, eBioscience™

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

13-1001-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.125 µg/test
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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BMA12 (BMA-12)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603181

Product Specific Information

Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.

Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD100; M-Sema G; Sema J; semaphorin H; Semaphorin-4D; Semaphorin-C-like 2; Semaphorin-J

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Gene Aliases: CD100; coll-4; Sema4d; Semacl2; Semaj; Semcl2

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UniProt ID: (Mouse) O09126

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Entrez Gene ID: (Mouse) 20354

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