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Invitrogen
CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD100 (SEMA4D) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD100 (SEMA4D) Antibody (14-1001-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD100 (SEMA4D) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD100 (SEMA4D) Antibody (14-1001-82) in Flow")
Product Details
14-1001-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
BMA12 (BMA-12)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1603189
Product Specific Information
Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72. Interaction of these two proteins diminishes the negative signaling normally effected by CD72. CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types. Antigen expression has been reported in Spleen, thymus and lymph nodes.
Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported for use in neutralization of mouse CD100. (Please use Functional Grade purified BMA12, Product # 16-1001, in functional assays).
Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD100; M-Sema G; Sema J; semaphorin H; Semaphorin-4D; Semaphorin-C-like 2; Semaphorin-J
Gene Aliases: CD100; coll-4; Sema4d; Semacl2; Semaj; Semcl2
UniProt ID: (Mouse) O09126
Entrez Gene ID: (Mouse) 20354
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
negative regulation of cell adhesion
multicellular organism development
nervous system development
regulation of cell shape
negative regulation of alkaline phosphatase activity
positive regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
positive regulation of cell migration
regulation of cell projection organization
positive regulation of GTPase activity
ossification involved in bone maturation
negative regulation of osteoblast differentiation
positive regulation of collateral sprouting
regulation of dendrite morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of peptidyl-tyrosine phosphorylation
positive regulation of axonogenesis
leukocyte aggregation
semaphorin-plexin signaling pathway
semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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