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Invitrogen
CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), eBioscience™
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FIGURE: 1 / 5
CD103 (Integrin alpha E) Antibody (14-1038-82) in Flow
Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
Product Details
14-1038-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
B-Ly7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467412
Product Specific Information
Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.
Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: The B-Ly7 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD103, also known as ITGAE or Integrin alpha E, is a molecule that non-covalently associates with integrin beta 7 to form a heterodimeric integral membrane protein. This protein is part of the integrin family, characterized by an alpha chain and a beta chain. Specifically, CD103 includes a 150 kDa alpha chain and a 120 kDa beta chain, with the alpha integrin undergoing post-translational cleavage in the extracellular domain to yield disulfide-linked heavy and light chains. CD103 is primarily expressed on mucosa-associated T lymphocytes, intraepithelial lymphocytes (IELs), and activated cells, as well as a subset of TGF beta-1 cells. It is preferentially found in human intestinal IELs, where it plays a role in adhesion and may serve as an accessory molecule for IEL activation. CD103 binds to epithelial cell antigen E-cadherin, mediating the homing of lymphocytes to the intestinal epithelium. In peripheral blood and lymphoid organs, CD103 is expressed by 0.5 to 2% of resting lymphocytes, and its expression can be upregulated in vitro by stimulating human T cells with mitogens. CD103 is also expressed by hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. The CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; integrin alpha E; Integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); Intregrin alpha E2; MGC141996; Mucosal lymphocyte 1 antigen
Gene Aliases: CD103; HUMINAE; ITGAE
UniProt ID: (Human) P38570
Entrez Gene ID: (Human) 3682
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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