Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (OX62), PE, eBioscience™

1 Published Figure

6 References

View all (90) CD103 (Integrin alpha E) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD103 (Integrin alpha E) Antibody (12-1030-82) in Flow

Staining of rat splenocytes with Anti-Rat CD4 FITC (Product # 11-0040-82) and 0.125 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Rat CD103 (Integrin alpha E) PE (right). Cells in the lymphocyte gate were used for analysis and events are displayed after gating out CD3+ T cells and CD45RB+ cells and gating on the remaining cells, which are MHC Class II+.

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Product Details

12-1030-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX62

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1210757

Product Specific Information

Description: The OX62 monoclonal antibody reacts with rat CD103, also known as alphaE2 integrin. CD103 was originally identified on rat veiled dendritic cells, and is expressed by dendritic epidermal T cells in the skin, intraepithelial lymphocytes in the small intestine and by dendritic cells associated with gammadelta T cells. Integrins are a family of heterodimeric proteins which contain non-covalently associated alpha and beta transmembrane glycoproteins. Integrins function as receptors for extracellular matrix and cell-cell adhesion molecules.

Applications Reported: This OX62 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX62 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD103, also known as ITGAE or Integrin alpha E, is a molecule that non-covalently associates with integrin beta 7 to form a heterodimeric integral membrane protein. This protein is part of the integrin family, characterized by an alpha chain and a beta chain. Specifically, CD103 includes a 150 kDa alpha chain and a 120 kDa beta chain, with the alpha integrin undergoing post-translational cleavage in the extracellular domain to yield disulfide-linked heavy and light chains. CD103 is primarily expressed on mucosa-associated T lymphocytes, intraepithelial lymphocytes (IELs), and activated cells, as well as a subset of TGF beta-1 cells. It is preferentially found in human intestinal IELs, where it plays a role in adhesion and may serve as an accessory molecule for IEL activation. CD103 binds to epithelial cell antigen E-cadherin, mediating the homing of lymphocytes to the intestinal epithelium. In peripheral blood and lymphoid organs, CD103 is expressed by 0.5 to 2% of resting lymphocytes, and its expression can be upregulated in vitro by stimulating human T cells with mitogens. CD103 is also expressed by hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. The CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Resveratrol regulates macrophage recruitment and M1 macrophage polarization and prevents corneal allograft rejection in rats.

Frontiers in medicine

Xu C,Guo R,Hou C,Ma M,Dong X,Ouyang C,Wu J,Huang T

12-1030-82 was used in Flow Cytometry to suggest that resveratrol can reduce corneal macrophage recruitment and M1 macrophage polarization after corneal transplantation in rats and prevent CGR.

Thu Nov 09 00:00:00 UTC 2023

Cell release during perfusion reflects cold ischemic injury in rat livers.

Scientific reports

de Vries RJ,Pendexter CA,Cronin SEJ,Marques B,Hafiz EOA,Muzikansky A,van Gulik TM,Markmann JF,Stott SL,Yeh H,Toner M,Uygun K,Tessier SN

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 12-1030-82) in Flow Cytometry

Fri Jan 24 00:00:00 UTC 2020

Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats.

Molecular medicine reports

Li Y,Du Y,Zhang A,Jiang R,Nie X,Xiong X

12-1030-82 was used in Flow cytometry to investigate the effects of CC chemokine receptor 7 on Dendritic cells‚mediated immune tolerance in allergic asthma.

Fri Nov 01 00:00:00 UTC 2019

Amelioration of collagen-induced arthritis using antigen-loaded dendritic cells modified with NF-κB decoy oligodeoxynucleotides.

Drug design, development and therapy

Jiang H,Hu H,Zhang Y,Yue P,Ning L,Zhou Y,Shi P,Yuan R

12-1030 was used in Flow cytometry/Cell sorting to investigate the therapeutic effect of dendritic cells loaded with bovine type II collagen on collagen-induced arthritis in rats.

Mon Jul 23 00:00:00 UTC 2018

Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model.

Cancer science

Inoue K,Saegusa N,Omiya M,Ashizawa T,Miyata H,Komiyama M,Iizuka A,Kume A,Sugino T,Yamaguchi K,Kiyohara Y,Nakagawa M,Akiyama Y

12-1030 was used in Flow cytometry/Cell sorting to suggest that a novel immunotherapy based on the immuno-flap method has the potential for clinical application to prevent the local recurrence of head and neck cancer patients.

Sun Feb 01 00:00:00 UTC 2015

Interleukin 23 production by intestinal CD103(+)CD11b(+) dendritic cells in response to bacterial flagellin enhances mucosal innate immune defense.

Immunity

Kinnebrew MA,Buffie CG,Diehl GE,Zenewicz LA,Leiner I,Hohl TM,Flavell RA,Littman DR,Pamer EG

12-1030 was used in Flow cytometry/Cell sorting to demonstrate cytokine expression by certain DCs drives IL-22-dependent RegIIIγ production for protection from bacterial colonisation.

Fri Feb 24 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: DC; DCs; integrin alpha E1, epithelial-associated; integrin alpha-E; integrin, alpha E, epithelial-associated; Intregrin alpha E2; MGC141996

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Entrez Gene ID: (Rat) 83577