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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD105 (Endoglin) Antibodies

          Invitrogen

          CD105 (Endoglin) Monoclonal Antibody (SN6), PE, eBioscience™

          57 Published Figures
          92 References
          View all (127) CD105 (Endoglin) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD105 (Endoglin) Monoclonal Antibody (SN6), PE, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (57)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 58

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          CD105 (Endoglin) Antibody (12-1057-42) in Flow

          Staining of U937 cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD105 (Endoglin) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)
          CD105 (Endoglin) Antibody in Flow Cytometry (Flow)

          Product Details

          12-1057-42

          Applications
          Tested Dilution
          Publications

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          5 µL (1 µg)/test
          View 90 publications 90 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Bovine, Fruit fly, Horse, Human, Mammal, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SN6

          Conjugate

          PE PE PE
          • Unconjugated
          • APC 
          • APC-eFluor 780
          • Brilliant UV 661
          • eFluor 450
          • Functional Grade
          • PE-Cyanine7
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1311123

          Product Specific Information

          Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

          Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD105; CD105 antigen; Endoglin; ORW

          View more View less

          Gene Aliases: END; ENG; HHT1; ORW1

          View more View less

          UniProt ID: (Human) P17813

          View more View less

          Entrez Gene ID: (Human) 2022

          View more View less

          Function(s)
          transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding
          Process(es)
          chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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