Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), eFluor™ 450, eBioscience™

8 Published Figures

11 References

View all (94) VCAM-1 (CD106) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 9

CD106 (VCAM-1) Antibody (48-1061-82) in Flow

Staining of BALB/c bone marrow cells with 0.125 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.125 µg of Anti-Mouse CD106 (VCAM-1) eFluor® 450 (right). Cells in the large scatter population were used for analysis.

CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC)

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Product Details

48-1061-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

429

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1633378

Product Specific Information

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Sun Apr 17 00:00:00 UTC 2022

Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.

Cell death and differentiation

Xie SJ,Li JH,Chen HF,Tan YY,Liu SR,Zhang Y,Xu H,Yang JH,Liu S,Zheng LL,Huang MB,Guo YH,Zhang Q,Zhou H,Qu LH

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Sat Sep 01 00:00:00 UTC 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Tue Oct 16 00:00:00 UTC 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Tue Jun 21 00:00:00 UTC 2016

MicroRNA-155 Exerts Cell-Specific Antiangiogenic but Proarteriogenic Effects During Adaptive Neovascularization.

Circulation

Pankratz F,Bemtgen X,Zeiser R,Leonhardt F,Kreuzaler S,Hilgendorf I,Smolka C,Helbing T,Hoefer I,Esser JS,Kustermann M,Moser M,Bode C,Grundmann S

48-1061 was used in Flow cytometry/Cell sorting to identify regulatory microRNAs involved in the modulation of neovascularisation after femoral artery occlusion in mice.

Tue May 05 00:00:00 UTC 2015

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Mon Nov 16 00:00:00 UTC 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Flow Cytometry

Thu Mar 13 00:00:00 UTC 2014

Effects of intra-abdominal sepsis on atherosclerosis in mice.

Critical care (London, England)

Kaynar AM,Yende S,Zhu L,Frederick DR,Chambers R,Burton CL,Carter M,Stolz DB,Agostini B,Gregory AD,Nagarajan S,Shapiro SD,Angus DC

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Immunofluorescence

Wed Sep 03 00:00:00 UTC 2014

Rhodamine-loaded intercellular adhesion molecule-1-targeted microbubbles for dual-modality imaging under controlled shear stresses.

Circulation. Cardiovascular imaging

Wu Z,Curaj A,Fokong S,Liehn EA,Weber C,Lammers T,Kiessling F,Zandvoort van M

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 48-1061-82) in Immunohistochemistry

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; vascular cell adhesion protein 1

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Gene Aliases: CD106; Vcam-1

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Entrez Gene ID: (Mouse) 22329

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Function(s)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Process(es)

cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion heart development amine metabolic process viral process single organismal cell-cell adhesion positive regulation of T cell proliferation leukocyte tethering or rolling oxidation-reduction process cell chemotaxis embryonic placenta morphogenesis chorio-allantoic fusion visceral serous pericardium development cellular response to glucose stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD106 (VCAM-1) Monoclonal Antibody (429), eFluor™ 450, eBioscience™

CD106 (VCAM-1) Antibody (48-1061-82) in Flow

Product Details

48-1061-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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