Invitrogen

CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Alexa Fluor™ 488, eBioscience™

CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)

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CD107a (LAMP-1) Antibody (53-1071-82) in ICC/IF

Immunofluorescence analysis of LAMP1 (Lysosome-associated membrane glycoprotein 1) was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Alexa F... View More

CD107a (LAMP-1) Antibody in Western Blot (WB)

CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)

CD107a (LAMP-1) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

Product Details

53-1071-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100

View 8 publications 8 publications

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D4B (1D4B)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_657536

Product Specific Information

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.

Applications Reported: This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay.

Applications Tested: This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The immune and metabolic treatment approach of using testosterone on mice models of liver injury.

Frontiers in pharmacology

Amer J,Salhab A,Snobar H,Alhabil Y

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Fri Aug 25 00:00:00 UTC 2023

A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3.

Nature communications

Ta A,Ricci-Azevedo R,Vasudevan SO,Wright SS,Kumari P,Havira MS,Surendran Nair M,Rathinam VA,Vanaja SK

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Western Blot

Tue Apr 11 00:00:00 UTC 2023

P. gingivalis Infection Upregulates PD-L1 Expression on Dendritic Cells, Suppresses CD8+ T-cell Responses, and Aggravates Oral Cancer.

Cancer immunology research

Ren J,Han X,Lohner H,Hoyle RG,Li J,Liang S,Wang H

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2023

Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation.

BMC immunology

Benjamin SJ,Hawley KL,Vera-Licona P,La Vake CJ,Cervantes JL,Ruan Y,Radolf JD,Salazar JC

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunocytochemistry

Mon May 17 00:00:00 UTC 2021

A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis.

Cell death & disease

Wang X,Li Z,Bai Y,Zhang R,Meng R,Chen F,Wang H,Billiar TR,Xiao X,Lu B,Tang Y

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Western Blot

Wed Apr 14 00:00:00 UTC 2021

An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling.

Frontiers in pharmacology

Wang X,Shi J,Li Z,Li L,Zhang R,Bai Y,Li J,Liang F,Tang Y

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Western Blot

Tue Jun 08 00:00:00 UTC 2021

Synaptojanin1 deficiency upregulates basal autophagosome formation in astrocytes.

The Journal of biological chemistry

Pan PY,Zhu J,Rizvi A,Zhu X,Tanaka H,Dreyfus CF

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunocytochemistry

Thu Jul 01 00:00:00 UTC 2021

Immunotherapy with engineered bacteria by targeting the STING pathway for anti-tumor immunity.

Nature communications

Leventhal DS,Sokolovska A,Li N,Plescia C,Kolodziej SA,Gallant CW,Christmas R,Gao JR,James MJ,Abin-Fuentes A,Momin M,Bergeron C,Fisher A,Miller PF,West KA,Lora JM

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunocytochemistry

Mon Jun 01 00:00:00 UTC 2020

Macropinocytosis drives T cell growth by sustaining the activation of mTORC1.

Nature communications

Charpentier JC,Chen D,Lapinski PE,Turner J,Grigorova I,Swanson JA,King PD

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunofluorescence

Fri Jan 10 00:00:00 UTC 2020

Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.

Cell reports

Cham LB,Torrez Dulgeroff LB,Tal MC,Adomati T,Li F,Bhat H,Huang A,Lang PA,Moreno ME,Rivera JM,Galkina SA,Kosikova G,Stoddart CA,McCune JM,Myers LM,Weissman IL,Lang KS,Hasenkrug KJ

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Tue Apr 14 00:00:00 UTC 2020

25(OH) D3 alleviate liver NK cytotoxicity in acute but not in chronic fibrosis model of BALB/c mice due to modulations in vitamin D receptor.

BMC gastroenterology

Salhab A,Amer J,Yinying L,Safadi R

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Fri Apr 10 00:00:00 UTC 2020

Foam Cell Induction Activates AMPK But Uncouples Its Regulation of Autophagy and Lysosomal Homeostasis.

International journal of molecular sciences

LeBlond ND,Nunes JRC,Smith TKT,O'Dwyer C,Robichaud S,Gadde S,Côté M,Kemp BE,Ouimet M,Fullerton MD

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunocytochemistry

Fri Nov 27 00:00:00 UTC 2020

Leishmania amazonensis hijacks host cell lysosomes involved in plasma membrane repair to induce invasion in fibroblasts.

Journal of cell science

Cavalcante-Costa VS,Costa-Reginaldo M,Queiroz-Oliveira T,Oliveira ACS,Couto NF,Dos Anjos DO,Lima-Santos J,Andrade LO,Horta MF,Castro-Gomes T

53-1071 was used in Immunocytochemistry-immunoflourescence to show that L. amazonensis can induce their own entry into fibroblasts independently of actin cytoskeleton activity, and, thus, through a mechanism that is distinct from phagocytosis.

Mon Mar 25 00:00:00 UTC 2019

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Oncology reports

Li Y,Zhao C,Liu J,Lu Z,Lu M,Gu J,Liu R

53-1071 was used in Flow cytometry/Cell sorting to investigate whether CD1d expression on dendritic cells is capable of enhancing anti-tumour immunity, particularly T-cell related immunity.

Wed May 01 00:00:00 UTC 2019

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

53-1071 was used in Flow cytometry/Cell sorting to study polysaccharide A as an immunomodulatory symbiosis factor whcih coordinates anti-inflammatory responses during viral infections.

Tue May 14 00:00:00 UTC 2019

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Wed Mar 28 00:00:00 UTC 2018

A TLR7 agonist enhances the antitumor efficacy of obinutuzumab in murine lymphoma models via NK cells and CD4 T cells.

Leukemia

Cheadle EJ,Lipowska-Bhalla G,Dovedi SJ,Fagnano E,Klein C,Honeychurch J,Illidge TM

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2017

Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection.

The Journal of experimental medicine

Qi X,Man SM,Malireddi RK,Karki R,Lupfer C,Gurung P,Neale G,Guy CS,Lamkanfi M,Kanneganti TD

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunofluorescence

Mon Sep 19 00:00:00 UTC 2016

Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection.

The Journal of experimental medicine

Qi X,Man SM,Malireddi RK,Karki R,Lupfer C,Gurung P,Neale G,Guy CS,Lamkanfi M,Kanneganti TD

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunofluorescence

Mon Sep 19 00:00:00 UTC 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Wed Sep 07 00:00:00 UTC 2016

Intrahepatic innate lymphoid cells secrete IL-17A and IL-17F that are crucial for T cell priming in viral infection.

Journal of immunology (Baltimore, Md. : 1950)

Jie Z,Liang Y,Hou L,Dong C,Iwakura Y,Soong L,Cong Y,Sun J

53-1071 was used in Flow cytometry/Cell sorting to investigate the source and regulation of the IL-17 surge in the liver microenvironment that occurs early in the response to viral infection.

Tue Apr 01 00:00:00 UTC 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Flow Cytometry

Mon Jul 06 00:00:00 UTC 2015

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

53-1071 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 UTC 2011

The NALP3 inflammasome is involved in the innate immune response to amyloid-beta.

Nature immunology

Halle A,Hornung V,Petzold GC,Stewart CR,Monks BG,Reinheckel T,Fitzgerald KA,Latz E,Moore KJ,Golenbock DT

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 53-1071-82) in Immunofluorescence

Fri Aug 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LGP-120; LGP-A; lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; P2B

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Gene Aliases: AI196048; CD107a; Lamp-1; Lamp1

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UniProt ID: (Mouse) P11438

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Entrez Gene ID: (Mouse) 16783

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Function(s)

protein binding enzyme binding protein domain specific binding

Process(es)

spermatogenesis granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD107a (LAMP-1) Monoclonal Antibody (eBio1D4B (1D4B)), Alexa Fluor™ 488, eBioscience™

CD107a (LAMP-1) Antibody (53-1071-82) in ICC/IF

Product Details

53-1071-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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