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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • LAMP1 (CD107a) Antibodies

          Invitrogen

          CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience™

          26 Published Figures
          34 References
          View all (87) LAMP1 (CD107a) antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), FITC, eBioscience™

          • Antibody Testing Data (2)
          • Published Figures (26)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 28

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          CD107a (LAMP-1) Antibody (11-1079-42) in Flow

          Intracellular staining of the Jurkat cell line with 0.5 µg of Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (open histogram) or 0.5 µg of Anti-Human CD107a (LAMP-1) FITC (filled histogram). Total cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunocytochemistry (ICC/IF)
          CD107a (LAMP-1) Antibody in Immunohistochemistry (IHC)
          CD107a (LAMP-1) Antibody in Western Blot (WB)

          Product Details

          11-1079-42

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          0.5 µg/mL
          View 8 publications 8 publications

          Flow Cytometry (Flow)

          5 µL (0.5 µg)/test
          View 23 publications 23 publications

          Inhibition Assays (IA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rhesus monkey

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBioH4A3

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Alexa Fluor 488 
          • Brilliant Violet 421
          • eFluor 450
          • eFluor 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10853196

          Product Specific Information

          Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.

          Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay.

          Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1

          View more View less

          Gene Aliases: CD107a; LAMP1; LAMPA; LGP120

          View more View less

          UniProt ID: (Human) P11279

          View more View less

          Entrez Gene ID: (Human) 3916

          View more View less

          Function(s)
          virus receptor activity protein binding enzyme binding protein domain specific binding
          Process(es)
          autophagy granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity viral entry into host cell autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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