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CD11B/Integrin alpha M Polyclonal Antibody

CD11B/Integrin alpha M Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD11B/Integrin alpha M Antibody (21851-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

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Product Details

21851-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

View 10 publications 10 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD11B/Integrin alpha M Fusion Protein Ag16327 (127-320 aa encoded by BC096346)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GSNLRQQPQ KFPEALRGCP QEDSDIAFLI DGSGSIIPHD FRRMKEFVST VMEQLKKSKT LFSLMQYSEE FRIHFTFKEF QNNPNPRSLV KPITQLLGRT HTATGIRKVV RELFNITNGA RKNAFKILVV ITDGEKFGDP LGYEDVIPEA DREGVIRYVI GVGDAFRSEK SRQELNTIAS KPPRDHVFQV NNFEA (127-320 aa encoded by BC096346)

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Human) 3684

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Function(s)

glycoprotein binding protein binding metal ion binding

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11B/Integrin alpha M Polyclonal Antibody

CD11B/Integrin alpha M Antibody (21851-1-AP) in IHC (P)

Product Details

21851-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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