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Invitrogen
CD11a Monoclonal Antibody (121/7)
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FIGURE: 1 / 2
CD11a Antibody (MA11A5) in Flow
Flow cytometry analysis of CD11a in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA11A5) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLigh... View More
Product Details
MA11A5
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
121/7
Immunogen
Mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_223586
Product Specific Information
MA11A5 targets CD11a in FACS, IA, and Neu applications and shows reactivity with mouse samples. This antibody does not react with human Jurkat cells in FACS applications.
The MA11A5 immunogen is mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1.
MA11A5 detects CD11a which has a predicted molecular weight of approximately 126 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Target Information
CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha L integrin; antigen CD11A p180; CD11 antigen-like family member A; CD11a; Integrin alpha-L; integrin alphaL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide
Gene Aliases: (p180); Cd11a; Itgal; LFA-1; LFA-1A; Ly-15; Ly-21
UniProt ID: (Mouse) P24063
Entrez Gene ID: (Mouse) 16408
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
signal transduction
cell surface receptor signaling pathway
integrin-mediated signaling pathway
single organismal cell-cell adhesion
regulation of cell-cell adhesion
positive regulation of cell-cell adhesion
positive regulation of T cell proliferation
receptor clustering
activated T cell proliferation
positive regulation of calcium-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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