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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD11b Antibodies

          Invitrogen

          CD11b Monoclonal Antibody (ICRF44), eBioscience™

          36 Published Figures
          56 References
          View all (219) CD11b antibodies

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          Datasheet
          Protocols
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          Datasheet
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          Cite CD11b Monoclonal Antibody (ICRF44), eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (36)
          CD11b Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD11b Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 37

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          CD11b Antibody (14-0118-82) in Flow

          Staining of normal human peripheral blood cells with Anti-Human CD11b PE. Autofluorescence is indicated by open histogram. Cells in the monocyte population were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Western Blot, Flow Cytometry (WB, Flow)
          CD11b Antibody in Western Blot, Flow Cytometry (WB, Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)

          Product Details

          14-0118-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          -

          Immunocytochemistry (ICC/IF)

          -
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          1 µg/test
          View 45 publications 45 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          Assay-Dependent
          -

          Functional Assay (Functional)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          ICRF44

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • eFluor 506
          • FITC
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 800
          • NovaFluor Red 710
          • NovaFluor Red 725
          • NovaFluor Red 755
          • NovaFluor Violet 690
          • NovaFluor Violet 800
          • NovaFluor Yellow 570
          • NovaFluor Yellow 590
          • NovaFluor Yellow 660
          • NovaFluor Yellow 755
          • NovaFluor Yellow 810
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_467120

          Product Specific Information

          Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

          Applications Reported: The ICRF44 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining on formaldehyde fixed tissue. ICRF44 has also been reported in inhibition of aggregation of PMNs.

          Applications Tested: The ICRF44 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; integrin alpha M; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; uncharacterized LOC101866035

          View more View less

          Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

          View more View less

          UniProt ID: (Human) P11215

          View more View less

          Entrez Gene ID: (Human) 3684, (Chimpanzee) 454069, (Cynomolgus monkey) 101866035

          View more View less

          Function(s)
          glycoprotein binding protein binding metal ion binding
          Process(es)
          cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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