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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD11b Antibodies

          Invitrogen

          CD11b Monoclonal Antibody (M1/70), Super Bright™ 702, eBioscience™

          89 Published Figures
          147 References
          View all (219) CD11b antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD11b Monoclonal Antibody (M1/70), Super Bright™ 702, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (89)
          CD11b Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD11b Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 90

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          CD11b Antibody (67-0112-82) in Flow

          Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b kappa Isotype Control Super Bright 702 (Product # 67-4031-82) (left) or Anti-Mouse CD11b Super Bright 702 (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunocytochemistry (ICC/IF)
          CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD11b Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (IHC)
          CD11b Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)
          CD11b Antibody in Flow Cytometry (Flow)

          Product Details

          67-0112-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 13 publications 13 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 4 publications 4 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 120 publications 120 publications

          Neutralization (Neu)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2b, kappa

          Recommended Isotype Control

          Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 702, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          M1/70

          Conjugate

          Super Bright™ 702 Super Bright™ 702 Super Bright™ 702
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 506
          • eFluor 660
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 585
          • NovaFluor Blue 610-30S
          • NovaFluor Blue 660-40S
          • NovaFluor Yellow 570
          • NovaFluor Yellow 590
          • NovaFluor Yellow 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          413/702 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2662389

          Product Specific Information

          Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

          Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse splenocyte or bone marrow cell suspensions. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

          View more View less

          Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

          View more View less

          Entrez Gene ID: (Mouse) 16409

          View more View less

          Function(s)
          opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity
          Process(es)
          leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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