Invitrogen

CD11b/c Monoclonal Antibody (OX42), PerCP-eFluor™ 710, eBioscience™

CD11b/c Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD11b/c Antibody (46-0110-82) in Flow

Staining of rat splenocytes with Anti-Rat Granulocyte Marker FITC (Product # 11-0570-82) and 0.06 µg of Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (left) or 0.06 µg of Anti-Rat CD11b/c PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-0110-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX42

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10735261

Product Specific Information

Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4). CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.

The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages. Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation.

Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.

Molecular medicine reports

Feng S,Wang S,Sun S,Su H,Zhang L

Published figure using CD11b/c monoclonal antibody (Product # 46-0110-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

Published figure using CD11b/c monoclonal antibody (Product # 46-0110-82) in Flow Cytometry

Tue Sep 28 00:00:00 UTC 2021

Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.

Scientific reports

Luck B,Engevik MA,Ganesh BP,Lackey EP,Lin T,Balderas M,Major A,Runge J,Luna RA,Sillitoe RV,Versalovic J

Published figure using CD11b/c monoclonal antibody (Product # 46-0110-82) in Flow Cytometry

Fri May 08 00:00:00 UTC 2020

Genetic deletion of microglial Panx1 attenuates morphine withdrawal, but not analgesic tolerance or hyperalgesia in mice.

Channels (Austin, Tex.)

Burma NE,Leduc-Pessah H,Trang T

Published figure using CD11b/c monoclonal antibody (Product # 46-0110-82) in Flow Cytometry

Sun Sep 03 00:00:00 UTC 2017

Effects of prenatal low protein and postnatal high fat diets on visceral adipose tissue macrophage phenotypes and IL-6 expression in Sprague Dawley rat offspring.

PloS one

Xie L,Zhang K,Rasmussen D,Wang J,Wu D,Roemmich JN,Bundy A,Johnson WT,Claycombe K

46011082 was used in flow cytometry to determine whether maternal undernutrition by protein restriction affects the adipose tissue macrophage M1 or M2 phenotypes under postnatal high fat diet

Wed Aug 09 00:00:00 UTC 2017

Methamphetamine decreases CD4 T cell frequency and alters pro-inflammatory cytokine production in a model of drug abuse.

European journal of pharmacology

Mata MM,Napier TC,Graves SM,Mahmood F,Raeisi S,Baum LL

46-0110 was used in Flow cytometry/Cell sorting to investigate the effect of methamphetamine on the systemic immune system.

Sun Apr 05 00:00:00 UTC 2015

Targeting glioblastoma with NK cells and mAb against NG2/CSPG4 prolongs animal survival.

Oncotarget

Poli A,Wang J,Domingues O,Planagumà J,Yan T,Rygh CB,Skaftnesmo KO,Thorsen F,McCormack E,Hentges F,Pedersen PH,Zimmer J,Enger PØ,Chekenya M

46-0110 was used in Flow cytometry/Cell sorting to investigate the therapeutic efficacy of combining adoptively transferred activated natural killer cells with a monoclonal antibody in glioblastoma-bearing rats.

Sun Sep 01 00:00:00 UTC 2013

Suppression of autoimmune arthritis by Celastrus-derived Celastrol through modulation of pro-inflammatory chemokines.

Bioorganic & medicinal chemistry

Venkatesha SH,Astry B,Nanjundaiah SM,Yu H,Moudgil KD

46-0110 was used in Flow cytometry/Cell sorting to investigate the mechanism of action of Celastrol on arthritis.

Sat Sep 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD11bc; CR3A; Integrin alpha M; integrin alpha-M; integrin alpha-X; integrin, alpha X; MAC-1; MAC1A; MGC117044; MO1A; OX-42; OX42; SLEB6

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Gene Aliases: Cd11b; RGD1561123

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Entrez Gene ID: (Rat) 25021, (Rat) 499271

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Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity

Process(es)

cell adhesion integrin-mediated signaling pathway heterotypic cell-cell adhesion activated T cell proliferation defense response to virus leukocyte migration involved in inflammatory response leukocyte cell-cell adhesion ectodermal cell differentiation microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation

Performance Guarantee

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CD11b/c Monoclonal Antibody (OX42), PerCP-eFluor™ 710, eBioscience™

CD11b/c Antibody (46-0110-82) in Flow

Product Details

46-0110-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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