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          • Primary Antibodies ›
          • CD11c Antibodies

          Invitrogen

          CD11c Monoclonal Antibody (N418), eFluor™ 506, eBioscience™

          85 Published Figures
          133 References
          View all (154) CD11c antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD11c Monoclonal Antibody (N418), eFluor™ 506, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (85)
          CD11c Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD11c Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 86

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          CD11c Antibody (69-0114-82) in Flow

          Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.125 µg of Armenian Hamster IgG Isotype Control eFluor® 506 (Product # 69-4888-82) (left) or 0.125 µg of Anti-Mouse CD11c eFluor® 506 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunocytochemistry (ICC/IF)
          CD11c Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          CD11c Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11c Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11c Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11c Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Immunohistochemistry (IHC)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)
          CD11c Antibody in Flow Cytometry (Flow)

          Product Details

          69-0114-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 19 publications 19 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 104 publications 104 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 506, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          N418

          Conjugate

          eFluor™ 506 eFluor™ 506 eFluor™ 506
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 650
          • Brilliant Violet 711
          • eFluor 450
          • eFluor 615
          • eFluor 660
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 610-30S
          • NovaFluor Blue 660-40S
          • NovaFluor Blue 690
          • NovaFluor Yellow 570
          • PE 
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          419/508 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2637427

          Product Specific Information

          Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

          Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

          Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement receptor 4; Integrin alpha-X; integrin aX; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95

          View more View less

          Gene Aliases: AI449405; Cd11c; Cr4; Itgax; N418

          View more View less

          UniProt ID: (Mouse) Q9QXH4

          View more View less

          Entrez Gene ID: (Mouse) 16411

          View more View less

          Function(s)
          metal ion binding
          Process(es)
          cell adhesion integrin-mediated signaling pathway heterotypic cell-cell adhesion activated T cell proliferation defense response to virus positive regulation of protein targeting to mitochondrion
          It has to be done as per old AB suggested Products section.
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