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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD133 (Prominin-1) Antibodies

          Invitrogen

          CD133 Monoclonal Antibody (5E3)

          Advanced Verification
          1 Reference
          View all (35) CD133 (Prominin-1) antibodies

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          Cite CD133 Monoclonal Antibody (5E3)

          • Antibody Testing Data (4)
          • Advanced Verification (2)
          CD133 Antibody in Western Blot (WB)
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          CD133 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          CD133 Antibody (MA5-18323) in WB

          Western Blot was performed using Anti-CD133 Monoclonal Antibody (5E3) (Product # MA5-18323) and a 133 kDa band corresponding to Prominin-1 was observed across cell lines tested except HeLa and SK-O-V3 which are reported to be low or negative for CD133 expression. Membrane enriched extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), Caco-2 (Lane 2), HT-29 (Lane 3), HeLa (Lane 4) and SK-O-V3 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD133 Antibody in Western Blot (WB)
          CD133 Antibody in Western Blot (WB)
          CD133 Antibody in Western Blot (WB)
          CD133 Antibody in Western Blot (WB)
          CD133 Antibody
          CD133 Antibody

          Product Details

          MA5-18323

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Mouse

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          5E3

          Immunogen

          Recombinant full length human CD133
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539686

          Product Specific Information

          This antibody detects endogenous protein at a molecular weight of 133 kDa and does not cross-react with related proteins.

          Purity is >95% by SDS-PAGE.

          Target Information

          The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Antigen AC133; antigen CD133; CD107b; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; Prominin-1; Prominin-like protein 1; Prominin1

          View more View less

          Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4

          View more View less

          UniProt ID: (Human) O43490

          View more View less

          Entrez Gene ID: (Human) 8842

          View more View less

          Function(s)
          protein binding actinin binding cadherin binding
          Process(es)
          retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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