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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD133 (Prominin-1) Antibodies

          Invitrogen

          CD133 (Prominin-1) Monoclonal Antibody (13A4), eBioscience™

          Advanced Verification
          49 Published Figures
          85 References
          View all (29) CD133 (Prominin-1) antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite CD133 (Prominin-1) Monoclonal Antibody (13A4), eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (49)
          • Advanced Verification (1)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 51

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          CD133 (Prominin-1) Antibody (14-1331-82) in Flow

          Staining of BALB/c bone marrow cells with 0.5 µg of Rat IgG1 kappa Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.5 µg of Anti-Mouse CD133 (Prominin-1) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the myeloid gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD133 (Prominin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD133 (Prominin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD133 (Prominin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD133 (Prominin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD133 (Prominin-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Immunohistochemistry (IHC)
          CD133 (Prominin-1) Antibody in Western Blot (WB)
          CD133 (Prominin-1) Antibody in Western Blot (WB)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody in Flow Cytometry (Flow)
          CD133 (Prominin-1) Antibody

          Product Details

          14-1331-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          View 10 publications 10 publications

          Immunohistochemistry (IHC)

          -
          View 29 publications 29 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          View 5 publications 5 publications

          Immunocytochemistry (ICC/IF)

          -
          View 14 publications 14 publications

          Flow Cytometry (Flow)

          1 µg/test
          View 25 publications 25 publications

          Immunoprecipitation (IP)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Dog, Mouse

          Published species

          Dog, Human, Mouse, Rat

          Host/Isotype

          Rat / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          13A4

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • APC 
          • Biotin 
          • FITC
          • PE 
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 702
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_467471

          Product Specific Information

          Description: The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen but has been reported to work in canine/dog.

          Applications Reported: The 13A4 antibody has been reported for use in flow cytometric analysis (~0.25-1 µg/million cells), immunoprecipitation (~10-25 µg/mL), immunoblotting (WB) (~1-5 µg/mL), and immunohistochemical staining (~1-10 µg/mL).

          Applications Tested: The 13A4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Antigen AC133 homolog; antigen CD133; CD107b; CD133; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; prominin 1.s1; Prominin-1; prominin-like 1; Prominin-like protein 1; Prominin1

          View more View less

          Gene Aliases: 4932416E19Rik; AC133; CD133; Prom; Prom-1; Prom1; Proml1

          View more View less

          UniProt ID: (Mouse) O54990

          View more View less

          Entrez Gene ID: (Dog) 488816, (Mouse) 19126

          View more View less

          Function(s)
          actinin binding cadherin binding
          Process(es)
          retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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