Invitrogen

CD134 (OX40) Monoclonal Antibody (OX-86), Functional Grade, eBioscience™

4 Published Figures

12 References

View all (83) OX40 (CD134) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD134 (OX40) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD134 (OX40) Antibody (16-1341-85) in Flow

Staining of Con A stimulated mouse splenocytes with Anti-Mouse CD134 (OX40) PE.Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

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Product Details

16-1341-85

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 7 publications 7 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX-86

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469064

Product Specific Information

Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.

Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis. It does not block binding of CD134 to its ligand, but has been reported for use in functional assays.

Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry

Tue Jun 08 00:00:00 UTC 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry

Wed Jul 14 00:00:00 UTC 2021

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry

Tue Aug 15 00:00:00 UTC 2017

Late stages of T cell maturation in the thymus involve NF-κB and tonic type I interferon signaling.

Nature immunology

Xing Y,Wang X,Jameson SC,Hogquist KA

16-1341 was used in Flow cytometry/Cell sorting to discover that both NF-κB and tonic interferon signals are involved in the final maturation of thymocytes into naive T cells.

Sun May 01 00:00:00 UTC 2016

Age-dependent divergent effects of OX40L treatment on the development of diabetes in NOD mice.

Autoimmunity

Haddad CS,Bhattacharya P,Alharshawi K,Marinelarena A,Kumar P,El-Sayed O,Elshabrawy HA,Epstein AL,Prabhakar BS

Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.

Nature immunology

Mahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA

16-1341 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells..

Thu May 01 00:00:00 UTC 2014

Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.

Mucosal immunology

Yokota-Nakatsuma A,Takeuchi H,Ohoka Y,Kato C,Song SY,Hoshino T,Yagita H,Ohteki T,Iwata M

16-1341 was used in Neutralization experiments to investigate the role of retinoic acid in mesenteric lymph node dendritic cells.

Tue Jul 01 00:00:00 UTC 2014

The number of responding CD4 T cells and the dose of antigen conjointly determine the TH1/TH2 phenotype by modulating B7/CD28 interactions.

Journal of immunology (Baltimore, Md. : 1950)

Rudulier CD,McKinstry KK,Al-Yassin GA,Kroeger DR,Bretscher PA

16-1341 was used in Functional assays to show that a greater number of Ag-dependent CD4 T cell interactions are required to generate Th2 than Th1 cells.

Sun Jun 01 00:00:00 UTC 2014

TNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40.

European journal of immunology

Hamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen X

16-1341 was used in Functional assays to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40.

Fri Jul 01 00:00:00 UTC 2011

Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.

Cancer research

Andarini S,Kikuchi T,Nukiwa M,Pradono P,Suzuki T,Ohkouchi S,Inoue A,Maemondo M,Ishii N,Saijo Y,Sugamura K,Nukiwa T

Sat May 01 00:00:00 UTC 2004

IL-17 production from activated T cells is required for the spontaneous development of destructive arthritis in mice deficient in IL-1 receptor antagonist.

Proceedings of the National Academy of Sciences of the United States of America

Nakae S,Saijo S,Horai R,Sudo K,Mori S,Iwakura Y

Published figure using CD134 (OX40) monoclonal antibody (Product # 16-1341-85) in Flow Cytometry

Tue May 13 00:00:00 UTC 2003

Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.

Journal of immunology (Baltimore, Md. : 1950)

Higgins LM,McDonald SA,Whittle N,Crockett N,Shields JG,MacDonald TT

Fri Jan 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: CD134; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; Tumor necrosis factor receptor superfamily member 4

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Gene Aliases: ACT35; CD134; Ly-70; Ox40; Tnfrsf4; Txgp1; TXGP1L

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UniProt ID: (Mouse) P47741

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Entrez Gene ID: (Mouse) 22163

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Function(s)

tumor necrosis factor-activated receptor activity protein binding

Process(es)

inflammatory response cellular defense response programmed cell death positive regulation of B cell proliferation tumor necrosis factor-mediated signaling pathway T cell proliferation regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase activity negative regulation of transcription, DNA-templated negative regulation of cytokine secretion positive regulation of immunoglobulin secretion negative regulation of activation-induced cell death of T cells negative regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD134 (OX40) Monoclonal Antibody (OX-86), Functional Grade, eBioscience™

CD134 (OX40) Antibody (16-1341-85) in Flow

Product Details

16-1341-85

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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