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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD14 Antibodies

          Invitrogen

          CD14 Monoclonal Antibody (61D3), eBioscience™

          Advanced Verification
          54 Published Figures
          90 References
          View all (206) CD14 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD14 Monoclonal Antibody (61D3), eBioscience™

          • Antibody Testing Data (3)
          • Published Figures (54)
          • Advanced Verification (1)
          CD14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          CD14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 58

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          CD14 Antibody (14-0149-82) in IHC (P)

          Immunohistochemistry of formalin-fixed paraffin embedded human lung tissue stained with 20 µg/mL of Anti-Human CD14 Purified, followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase and Fast Red. Nuclei are counterstained with hematoxylin. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD14 Antibody in Western Blot (WB)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Immunocytochemistry (ICC/IF)
          CD14 Antibody in Immunocytochemistry (ICC/IF)
          CD14 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD14 Antibody in Immunocytochemistry (ICC/IF)
          CD14 Antibody in Immunocytochemistry (ICC/IF)
          CD14 Antibody in Immunohistochemistry (IHC)
          CD14 Antibody in Immunohistochemistry (IHC)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Flow Cytometry (Flow)
          CD14 Antibody in Neutralization (Neu)
          CD14 Antibody

          Product Details

          14-0149-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          20 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 76 publications 76 publications

          Neutralization (Neu)

          -
          View 4 publications 4 publications

          Functional Assay (Functional)

          Assay-Dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          61D3

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 532 
          • Alexa Fluor 660 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 395
          • Brilliant UV 496
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant UV 805
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • eFluor 506
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 555
          • NovaFluor Blue 585
          • NovaFluor Blue 610-30S
          • NovaFluor Blue 660-40S
          • NovaFluor Blue 800
          • NovaFluor Yellow 570
          • NovaFluor Yellow 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_467129

          Product Specific Information

          Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

          Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded human tissue. 61D3 has also been reported for in vitro functional studies. (Please use Functional Grade Purified 61D3, Product # 16-0149, in functional assays, and fluorochrome-conjugated 61D3 is recommended for use in flow cytometry).

          Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 61D3 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

          View more View less

          Gene Aliases: CD14

          View more View less

          UniProt ID: (Human) P08571

          View more View less

          Entrez Gene ID: (Human) 929

          View more View less

          Function(s)
          lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding
          Process(es)
          MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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