Invitrogen

CD14 Monoclonal Antibody (TüK4), Qdot™ 605

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CD14 Antibody (Q10013) in Flow

Qdot® 605 anti-human CD14 conjugate 605/20 band pass filter

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Product Details

Q10013

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 15 publications 15 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TüK4

Immunogen

The CD14 antigen

Conjugate

Qdot™ 605 Qdot™ 605 Qdot™ 605

Excitation/Emission Max

300/603 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2556439

Product Specific Information

Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10014.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10013: peak excitation 405 (488) nm/peak emission 605 nm; recommended filter 605/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.

Vaccines

Sangare K,Helmold Hait S,Moore M,Hogge C,Hoang T,Rahman MA,Venzon DJ,LaBranche C,Montefiori D,Robert-Guroff M,Thomas MA

Q10013 was used in Flow Cytometry to show that cells infected with an E1B55K and E4orf1-deleted (ΔE41) Ad exhibited reduced levels of phosphorylated Akt (Ser473 and Thr308)) and expressed different intrinsic innate immune cytokines from those induced in cells infected with an E4orf1-containing, ΔE1B parental Ad that exhibited elevated levels of phosphorylated Akt.

Tue Feb 15 00:00:00 UTC 2022

Cytomegalovirus mediates expansion of IL-15-responsive innate-memory cells with SIV killing function.

The Journal of clinical investigation

Méndez-Lagares G,Chin N,Chang WLW,Lee J,Rosás-Umbert M,Kieu HT,Merriam D,Lu W,Kim S,Adamson L,Brander C,Luciw PA,Barry PA,Hartigan-O'Connor DJ

Q10013 was used in Flow Cytometry to investigate the effects of rhesus CMV (RhCMV) on composition and function of the immune system in young macaques.

Mon Aug 02 00:00:00 UTC 2021

Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer.

Cancer cell

Costa A,Kieffer Y,Scholer-Dahirel A,Pelon F,Bourachot B,Cardon M,Sirven P,Magagna I,Fuhrmann L,Bernard C,Bonneau C,Kondratova M,Kuperstein I,Zinovyev A,Givel AM,Parrini MC,Soumelis V,Vincent-Salomon A,Mechta-Grigoriou F

Q10013 was used in Flow cytometry/Cell sorting to study the role of carcinoma associated fibroblasts in the tumour microenvironment.

Mon Mar 12 00:00:00 UTC 2018

Metabolic Phenotypes of Response to Vaccination in Humans.

Cell

Li S,Sullivan NL,Rouphael N,Yu T,Banton S,Maddur MS,McCausland M,Chiu C,Canniff J,Dubey S,Liu K,Tran V,Hagan T,Duraisingham S,Wieland A,Mehta AK,Whitaker JA,Subramaniam S,Jones DP,Sette A,Vora K,Weinberg A,Mulligan MJ,Nakaya HI,Levin M,Ahmed R,Pulendran B

Q10013 was used in Flow cytometry/Cell sorting to study the mechanisms driving vaccinal responses, in healthy young and older adults immunised with the live attenuated shingles vaccine Zostavax.

Thu May 18 00:00:00 UTC 2017

Adjuvanting a Simian Immunodeficiency Virus Vaccine with Toll-Like Receptor Ligands Encapsulated in Nanoparticles Induces Persistent Antibody Responses and Enhanced Protection in TRIM5α Restrictive Macaques.

Journal of virology

Kasturi SP,Kozlowski PA,Nakaya HI,Burger MC,Russo P,Pham M,Kovalenkov Y,Silveira ELV,Havenar-Daughton C,Burton SL,Kilgore KM,Johnson MJ,Nabi R,Legere T,Sher ZJ,Chen X,Amara RR,Hunter E,Bosinger SE,Spearman P,Crotty S,Villinger F,Derdeyn CA,Wrammert J,Pulendran B

Q10013 was used in flow cytometry to study the efficacy of nanoparticle adjuvants for inducing protective immunity against simian immunodeficiency virus

Wed Feb 15 00:00:00 UTC 2017

Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection.

European journal of immunology

Venkatasubramanian S,Tripathi D,Tucker T,Paidipally P,Cheekatla S,Welch E,Raghunath A,Jeffers A,Tvinnereim AR,Schechter ME,Andrade BB,Mackman N,Idell S,Vankayalapati R

Q10013 was used in Flow cytometry/Cell sorting to determine the role of tissue factor expressed by myeloid cells during Mycobacterium tuberculosis infection.

Mon Feb 01 00:00:00 UTC 2016

Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Demers A,Shaw JM,Kang G,Ball D,Tuero I,Musich T,Mohanram V,Demberg T,Karpova TS,Li Q,Robert-Guroff M

Q10013 was used in Flow cytometry/Cell sorting to quantify SIV Env-specific IL-21-producing T follicular helper cells in response to vaccination.

Mon Feb 15 00:00:00 UTC 2016

Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques.

Journal of neuroimmunology

Cornwell WD,Wagner W,Lewis MG,Fan X,Rappaport J,Rogers TJ

Q10013 was used in Flow cytometry/Cell sorting to study the effect of chronic morphine administration on the circulating dendritic cell population dynamics associated with SIV infection.

Wed Jun 15 00:00:00 UTC 2016

An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions.

mAbs

Kinder M,Greenplate AR,Strohl WR,Jordan RE,Brezski RJ

Q10013 was used in flow cytometry to describe an Fc engineering approach that specifically affects antibody-dependent cytokine release

Tue Mar 22 00:00:00 UTC 2016

Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.

Bone marrow transplantation

Anandasabapathy N,Breton G,Hurley A,Caskey M,Trumpfheller C,Sarma P,Pring J,Pack M,Buckley N,Matei I,Lyden D,Green J,Hawthorne T,Marsh HC,Yellin M,Davis T,Keler T,Schlesinger SJ

Q10013 was used in flow cytometry to report the phase I trial results of CDX-301

Wed Jul 01 00:00:00 UTC 2015

Sequential staining improves detection of CCR2 and CX3CR1 on monocytes when simultaneously evaluating CCR5 by multicolor flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Jalbert E,Shikuma CM,Ndhlovu LC,Barbour JD

Q10013 was used in Flow cytometry/Cell sorting to evaluate and optimise the simultaneous detection of cell surface expression levels of CCR2, CX3CR1, and CCR5 in primary human monocytes using a single antibody panel.

Fri Mar 01 00:00:00 UTC 2013

Differential regulatory T cell activity in HIV type 1-exposed seronegative individuals.

AIDS research and human retroviruses

Pattacini L,Murnane PM,Kahle EM,Bolton MJ,Delrow JJ,Lingappa JR,Katabira E,Donnell D,McElrath MJ,Baeten JM,Lund JM

Q10013 was used in flow cytometry to determine the role of conventional and regulatory T cells in protection from HIV-1 infection.

Tue Oct 01 00:00:00 UTC 2013

Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria.

Journal of immunological methods

Shey MS,Hughes EJ,de Kock M,Barnard C,Stone L,Kollmann TR,Hanekom WA,Scriba TJ

Q10013 was used in Flow cytometry/Cell sorting to show that innate cell stimulation resulted in increased granularity of monocytes and mDC.

Tue Feb 28 00:00:00 UTC 2012

PLoS neglected tropical diseases

Nausch N,Louis D,Lantz O,Peguillet I,Trottein F,Chen IY,Appleby LJ,Bourke CD,Midzi N,Mduluza T,Mutapi F

Q10013 was used in flow cytometry to study of the age-related changes in human dendritic cells from subjects infected with Schistosoma haematobium

Mon Jan 28 00:00:00 UTC 2013

The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency.

The Journal of experimental medicine

Bigley V,Haniffa M,Doulatov S,Wang XN,Dickinson R,McGovern N,Jardine L,Pagan S,Dimmick I,Chua I,Wallis J,Lordan J,Morgan C,Kumararatne DS,Doffinger R,van der Burg M,van Dongen J,Cant A,Dick JE,Hambleton S,Collin M

Q10013 was used in Flow cytometry/Cell sorting to characterise the phenotypes of different immune compartments in various syndromes of congenital or acquired cellular deficiencies.

Mon Feb 14 00:00:00 UTC 2011

A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.

Immunology

Vargas-Inchaustegui DA,Demberg T,Robert-Guroff M

Q10013 was used in flow cytometry to characterize different macaque NK cell subpopulations.

Tue Nov 01 00:00:00 UTC 2011

Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry.

Journal of immunological methods

Kapoor V,Hakim FT,Rehman N,Gress RE,Telford WG

Q10013 was used in flow cytometry to study the improvement of simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry by quantum dots thermal stability

Fri May 15 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD14 Monoclonal Antibody (TüK4), Qdot™ 605

CD14 Antibody (Q10013) in Flow

Product Details

Q10013

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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