Invitrogen

CD14 Monoclonal Antibody (TuK4), PE-Cyanine5.5

6 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

CD14 Antibody (MHCD1418) in Flow

Human peripheral blood monocytes stained using PE-Cy®5.5 of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

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Product Details

MHCD1418

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 10% sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10371748

Product Specific Information

The R-phycoerythrin (PE)-Cy5.5 tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

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Product References

PD-1 directed immunotherapy alters Tfh and humoral immune responses to seasonal influenza vaccine.

Nature immunology

Herati RS,Knorr DA,Vella LA,Silva LV,Chilukuri L,Apostolidis SA,Huang AC,Muselman A,Manne S,Kuthuru O,Staupe RP,Adamski SA,Kannan S,Kurupati RK,Ertl HCJ,Wong JL,Bournazos S,McGettigan S,Schuchter LM,Kotecha RR,Funt SA,Voss MH,Motzer RJ,Lee CH,Bajorin DF,Mitchell TC,Ravetch JV,Wherry EJ

MHCD1418 was used in Flow cytometry/Cell sorting to examine the impact of anti-PD-1 immunotherapy on the Tfh-B cell axis responding to unrelated viral antigens.

Mon Aug 01 00:00:00 UTC 2022

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

Seyres D,Cabassi A,Lambourne JJ,Burden F,Farrow S,McKinney H,Batista J,Kempster C,Pietzner M,Slingsby O,Cao TH,Quinn PA,Stefanucci L,Sims MC,Rehnstrom K,Adams CL,Frary A,Ergüener B,Kreuzhuber R,Mocciaro G,D'Amore S,Koulman A,Grassi L,Griffin JL,Ng LL,Park A,Savage DB,Langenberg C,Bock C,Downes K,Wareham NJ,Allison M,Vacca M,Kirk PDW,Frontini M

MHCD1418 was used in Flow Cytometry to investigate cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature.

Sat Mar 12 00:00:00 UTC 2022

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

MHCD1418 was used in Flow Cytometry to investigate cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature.

Sat Mar 12 00:00:00 UTC 2022

Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition.

Cell

Pasciuto E,Burton OT,Roca CP,Lagou V,Rajan WD,Theys T,Mancuso R,Tito RY,Kouser L,Callaerts-Vegh Z,de la Fuente AG,Prezzemolo T,Mascali LG,Brajic A,Whyte CE,Yshii L,Martinez-Muriana A,Naughton M,Young A,Moudra A,Lemaitre P,Poovathingal S,Raes J,De Strooper B,Fitzgerald DC,Dooley J,Liston A

MHCD1418 was used in Flow cytometry/Cell sorting to investigate the function of brain-resident CD4 T cells.

Thu Aug 06 00:00:00 UTC 2020

Novel Markers of Angiogenesis in the Setting of Cognitive Impairment and Dementia.

Journal of Alzheimer's disease : JAD

Callahan CM,Apostolova LG,Gao S,Risacher SL,Case J,Saykin AJ,Lane KA,Swinford CG,Yoder MC

MHCD1418 was used in Flow cytometry/Cell sorting to explore the relationship between angiogenesis activity and evidence of neurodegeneration among older adults.

Mon May 10 00:00:00 UTC 2021

Differential transcriptome and development of human peripheral plasma cell subsets.

JCI insight

Garimalla S,Nguyen DC,Halliley JL,Tipton C,Rosenberg AF,Fucile CF,Saney CL,Kyu S,Kaminski D,Qian Y,Scheuermann RH,Gibson G,Sanz I,Lee FE

MHCD1418 was used in Flow cytometry/Cell sorting to study the heterogeneity of human antibody secreting cells after vaccination.

Thu May 02 00:00:00 UTC 2019

Effects of treatment of preweaning dairy calves with recombinant bovine somatotropin on immune responses and somatotropic axis.

Journal of dairy science

Belli AL,Reis RB,Veronese A,Moreira R,Flanagan K,Driver J,Nelson CD,Clapper JA,Ballou MA,Jeong KC,Chebel RC

MHCD1418 was used in Flow cytometry/Cell sorting to investigate the effects of treatment of pre-weaning calves with recombinant bovine somatotropin on the somatotropic axis, selected immune parameters, and hematology of calves around weaning.

Sun Jul 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

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CD14 Monoclonal Antibody (TuK4), PE-Cyanine5.5

CD14 Antibody (MHCD1418) in Flow

Product Details

MHCD1418

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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