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          • Primary Antibodies ›
          • VE-cadherin (CD144) Antibodies
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          Invitrogen

          CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™

          Advanced Verification
          42 Published Figures
          48 References
          View all (69) VE-cadherin (CD144) antibodies

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          Datasheet
          Protocols
          Datasheet
          Protocols

          Cite CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™

          • Antibody Testing Data (2)
          • Published Figures (42)
          • Advanced Verification (2)
          CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 46

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          CD144 (VE-cadherin) Antibody (14-1449-80) in ICC/IF

          Immunofluorescence analysis of VE-cadherin was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD144 (VE-cadherin) Monoclonal Antibody (16B1), eBioscience™ (Product # 14-1449-82) at 5 µg/mL in 0.1% BSA, in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)
          CD144 (VE-cadherin) Antibody in Western Blot (WB)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)
          CD144 (VE-cadherin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD144 (VE-cadherin) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CD144 (VE-cadherin) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CD144 (VE-cadherin) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CD144 (VE-cadherin) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          CD144 (VE-cadherin) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)
          CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)
          CD144 (VE-cadherin) Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)
          CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)
          CD144 (VE-cadherin) Antibody in Western Blot (WB)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)
          CD144 (VE-cadherin) Antibody
          CD144 (VE-cadherin) Antibody

          Product Details

          14-1449-80

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          Assay-Dependent
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 11 publications 11 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          View 21 publications 21 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication
          Product Specifications

          Published species

          Human, Mouse, Non-human primate

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          16B1

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          RRID

          AB_467494

          Product Specific Information

          Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

          Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), microscopy, and immunocytochemistry without fixation.

          Applications Tested: This 16B1 antibody has been tested by immunoblotting Human Umbilical Vein Endothelial Cell (HUVEC) lysates. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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