Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Biotin, eBioscience™

44 Published Figures

27 References

View all (56) VE-cadherin (CD144) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (44)

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 45

CD144 (VE-cadherin) Antibody (13-1441-82) in Flow

Staining of bEnd-3 cell line with 0.5 µg of Rat IgG1 K Isotype Control Biotin (Product # 13-4301-82) (blue histogram) or 0.125 µg of Anti-Mouse CD144 (VE-Cadherin) Biotin purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD144 (VE-cadherin) Antibody in Western Blot (WB)

CD144 (VE-cadherin) Antibody in Immunocytochemistry (ICC/IF)

CD144 (VE-cadherin) Antibody in Immunohistochemistry (IHC)

CD144 (VE-cadherin) Antibody in Flow Cytometry, ChIP Assay, Peptide array (Flow, ChIP, Array)

Product Image Gallery

Product Details

13-1441-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

1 µg/test

View 10 publications 10 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Peptide Array (Array)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioBV13 (BV13)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1234997

Product Specific Information

Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.25 µg This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Flow pattern-dependent mitochondrial dynamics regulates the metabolic profile and inflammatory state of endothelial cells.

JCI insight

Hong SG,Shin J,Choi SY,Powers JC,Meister BM,Sayoc J,Son JS,Tierney R,Recchia FA,Brown MD,Yang X,Park JY

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunohistochemistry

Thu Sep 22 00:00:00 UTC 2022

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Western Blot

Mon Nov 01 00:00:00 UTC 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Tue May 11 00:00:00 UTC 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Tue May 11 00:00:00 UTC 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Tue May 11 00:00:00 UTC 2021

VE-cadherin endocytosis controls vascular integrity and patterning during development.

The Journal of cell biology

Grimsley-Myers CM,Isaacson RH,Cadwell CM,Campos J,Hernandes MS,Myers KR,Seo T,Giang W,Griendling KK,Kowalczyk AP

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunocytochemistry

Mon May 04 00:00:00 UTC 2020

VE-cadherin endocytosis controls vascular integrity and patterning during development.

The Journal of cell biology

Grimsley-Myers CM,Isaacson RH,Cadwell CM,Campos J,Hernandes MS,Myers KR,Seo T,Giang W,Griendling KK,Kowalczyk AP

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunocytochemistry

Mon May 04 00:00:00 UTC 2020

Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis.

Frontiers in immunology

Fu H,Sun Y,Shao Y,Saredy J,Cueto R,Liu L,Drummer C,Johnson C,Xu K,Lu Y,Li X,Meng S,Xue ER,Tan J,Jhala NC,Yu D,Zhou Y,Bayless KJ,Yu J,Rogers TJ,Hu W,Snyder NW,Sun J,Qin X,Jiang X,Wang H,Yang X

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Fri Jul 09 00:00:00 UTC 2021

Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning.

eLife

Urata S,Iida T,Yamamoto M,Mizushima Y,Fujimoto C,Matsumoto Y,Yamasoba T,Okabe S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunohistochemistry

Fri Jan 18 00:00:00 UTC 2019

CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.

Nature neuroscience

Louveau A,Herz J,Alme MN,Salvador AF,Dong MQ,Viar KE,Herod SG,Knopp J,Setliff JC,Lupi AL,Da Mesquita S,Frost EL,Gaultier A,Harris TH,Cao R,Hu S,Lukens JR,Smirnov I,Overall CC,Oliver G,Kipnis J

13-1441 was used in Immunohistochemistry to demonstrate that meningeal lymphatics fulfill two critical criteria: they assist in the drainage of cerebrospinal fluid components and enable immune cells to enter draining lymph nodes in a CCR7-dependent manner.

Mon Oct 01 00:00:00 UTC 2018

CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.

Nature neuroscience

Louveau A,Herz J,Alme MN,Salvador AF,Dong MQ,Viar KE,Herod SG,Knopp J,Setliff JC,Lupi AL,Da Mesquita S,Frost EL,Gaultier A,Harris TH,Cao R,Hu S,Lukens JR,Smirnov I,Overall CC,Oliver G,Kipnis J

Mon Oct 01 00:00:00 UTC 2018

Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells.

Stem cell research & therapy

Park JK,Lee TW,Do EK,Moon HJ,Kim JH

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Wed Jul 18 00:00:00 UTC 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in ChIP assay

Tue Mar 20 00:00:00 UTC 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in ChIP assay

Tue Mar 20 00:00:00 UTC 2018

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.

eLife

Bergiers I,Andrews T,Vargel Bölükbaşı Ö,Buness A,Janosz E,Lopez-Anguita N,Ganter K,Kosim K,Celen C,Itır Perçin G,Collier P,Baying B,Benes V,Hemberg M,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in ChIP assay

Tue Mar 20 00:00:00 UTC 2018

Tumour ischaemia by interferon-γ resembles physiological blood vessel regression.

Nature

Kammertoens T,Friese C,Arina A,Idel C,Briesemeister D,Rothe M,Ivanov A,Szymborska A,Patone G,Kunz S,Sommermeyer D,Engels B,Leisegang M,Textor A,Fehling HJ,Fruttiger M,Lohoff M,Herrmann A,Yu H,Weichselbaum R,Uckert W,Hübner N,Gerhardt H,Beule D,Schreiber H,Blankenstein T

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunocytochemistry

Thu May 04 00:00:00 UTC 2017

EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta.

Scientific reports

Chen II,Caprioli A,Ohnuki H,Kwak H,Porcher C,Tosato G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Thu Jun 02 00:00:00 UTC 2016

Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development.

eLife

Ramo K,Sugamura K,Craige S,Keaney JF,Davis RJ

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Western Blot

Tue Aug 09 00:00:00 UTC 2016

Activation of the TGFβ pathway impairs endothelial to haematopoietic transition.

Scientific reports

Vargel Ö,Zhang Y,Kosim K,Ganter K,Foehr S,Mardenborough Y,Shvartsman M,Enright AJ,Krijgsveld J,Lancrin C

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Fri Feb 19 00:00:00 UTC 2016

Endothelial fibroblast growth factor receptor signaling is required for vascular remodeling following cardiac ischemia-reperfusion injury.

American journal of physiology. Heart and circulatory physiology

House SL,Castro AM,Lupu TS,Weinheimer C,Smith C,Kovacs A,Ornitz DM

13-1441-82 was used in Flow Cytometry to suggest the potential for therapeutic benefit from activation of endothelial FGFR pathways following ischemic injury to the heart.

Tue Mar 01 00:00:00 UTC 2016

Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions.

Genes & development

Zheng W,Nurmi H,Appak S,Sabine A,Bovay E,Korhonen EA,Orsenigo F,Lohela M,D'Amico G,Holopainen T,Leow CC,Dejana E,Petrova TV,Augustin HG,Alitalo K

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunofluorescence

Tue Jul 15 00:00:00 UTC 2014

Sox7 is regulated by ETV2 during cardiovascular development.

Stem cells and development

Behrens AN,Zierold C,Shi X,Ren Y,Koyano-Nakagawa N,Garry DJ,Martin CM

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Mon Sep 01 00:00:00 UTC 2014

The expression of Sox17 identifies and regulates haemogenic endothelium.

Nature cell biology

Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2013

HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells.

Cell communication and signaling : CCS

Weidemann A,Breyer J,Rehm M,Eckardt KU,Daniel C,Cicha I,Giehl K,Goppelt-Struebe M

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunofluorescence

Mon Oct 21 00:00:00 UTC 2013

ER71 specifies Flk-1+ hemangiogenic mesoderm by inhibiting cardiac mesoderm and Wnt signaling.

Blood

Liu F,Kang I,Park C,Chang LW,Wang W,Lee D,Lim DS,Vittet D,Nerbonne JM,Choi K

13-1441 was used in Flow cytometry/Cell sorting to investigate whether ER71 could modulate the haemangiogenic or cardiogenic outcome of the Flk-1(+) mesoderm.

Thu Apr 05 00:00:00 UTC 2012

Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Nakao S,Zandi S,Faez S,Kohno R,Hafezi-Moghadam A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunofluorescence

Wed Feb 01 00:00:00 UTC 2012

Endothelial-myofibroblast transition contributes to the early development of diabetic renal interstitial fibrosis in streptozotocin-induced diabetic mice.

The American journal of pathology

Li J,Qu X,Bertram JF

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 13-1441-82) in Immunofluorescence

Thu Oct 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: 7B4/cadherin-5; Cadherin-5; CD144; Vascular endothelial cadherin; VE-cadherin

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Gene Aliases: 7B4; AA408225; Cd144; Cdh5; VE-Cad; Vec; VEcad; VECD

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UniProt ID: (Mouse) P55284

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Entrez Gene ID: (Mouse) 12562

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Function(s)

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding metal ion binding

Process(es)

blood vessel maturation cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules negative regulation of cell proliferation single organismal cell-cell adhesion regulation of establishment of cell polarity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Biotin, eBioscience™

CD144 (VE-cadherin) Antibody (13-1441-82) in Flow

Product Details

13-1441-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Peptide Array (Array)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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