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CD146 Antibody (MA5-24142) in WB
Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using CD146 Monoclonal Antibody (OJ79) (Product # 35-7800), shows expression of protein in PC12 neuronal differentiated cell line as compared to undifferentiated PC-12.
Product Details
MA5-24142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
404722
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146 Arg19-Lys560
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605575
Product Specific Information
In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD146; Cell surface glycoprotein MUC18; Gicerin; l-gicerin; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18
Gene Aliases: CD146; Mcam; Muc18
UniProt ID: (Rat) Q9EPF2
Entrez Gene ID: (Rat) 78967
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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