Invitrogen

CD146 Monoclonal Antibody (C264), FITC

Product Details

MA5-28812

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/10^6 cells

Product Specifications

Species Reactivity

Avian, Chicken

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

C264

Immunogen

Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

<0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2745771

Target Information

CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is heavily glycosylated, with more than 50% of its mass derived from carbohydrates. CD146 is primarily expressed on endothelial cells at cell-cell junctions, playing a crucial role in cell adhesion and the cohesion of the endothelial monolayer in vascular tissue. In addition to endothelial cells, CD146 is expressed on bone marrow fibroblasts, mesenchymal stromal cells, endometrial stromal cells, and some tumors, particularly melanoma. It has also been observed on a subset of circulating T cells and activated lymphocytes, but not on circulating endothelial cells, as indicated by the absence of other endothelial markers such as CD31 and CD51/61. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule. It is involved in the recruitment of Fyn kinase and the subsequent tyrosine phosphorylation of intracellular proteins, which are important for actin cytoskeleton assembly. This signaling role includes triggering tyrosine phosphorylation of FYN and PTK2 and causing a transient increase in intracellular calcium concentration. In the context of cancer, CD146 expression may facilitate the interaction of melanoma cells with the vascular system, enhancing hematogenous tumor spread. Additionally, CD146 could serve as an adhesion molecule active in neural crest cells during embryonic development, highlighting its diverse roles in both normal physiological processes and disease states.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: cell surface glycoprotein MUC18; gicerin; HEMCAM

View more View less

Entrez Gene ID: (Chicken) 448832