Proteintech

CD146/MCAM Polyclonal Antibody

CD146/MCAM Antibody in Immunocytochemistry (ICC/IF)

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CD146/MCAM Antibody (17564-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CD146/MCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD146/MCAM Antibody in Western Blot (WB)

CD146/MCAM Antibody in Flow Cytometry (Flow)

CD146/MCAM Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17564-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:600

Immunocytochemistry (ICC/IF)

1:50-1:500

View 9 publications 9 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD146/MCAM Fusion Protein Ag11762 (26-350 aa encoded by BC056418)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GEAEQPAPE LVEVEVGSTA LLKCGLSQSQ GNLSHVDWFS VHKEKRTLIF RVRQGQGQSE PGEYEQRLSL QDRGATLALT QVTPQDERIF LCQGKRPRSQ EYRIQLRVYK APEEPNIQVN PLGIPVNSKE PEEVATCVGR NGYPIPQVIW YKNGRPLKEE KNRVHIQSSQ TVESSGLYTL QSILKAQLVK EDKDAQFYCE LNYRLPSGNH MKESREVTVP VFYPTEKVWL EVEPVGMLKE GDRVEIRCLA DGNPPPHFSI SKQNPSTREA EEETTNDNGV LVLEPARKEH SGRYECQGLD LDTMISLLSE PQELLVNYVS DVRVSP (26-350 aa encoded by BC056418)

Target Information

CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface.

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Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma.

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Chiu HY,Loh AHP,Taneja R

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Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

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Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

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Mon Oct 10 00:00:00 UTC 2022

Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

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Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis.

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Overexpression of MCAM induced by SMYD2-H3K36me2 in breast cancer stem cell properties.

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Pericyte mediates the infiltration, migration, and polarization of macrophages by CD163/MCAM axis in glioblastoma.

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Pericyte mediates the infiltration, migration, and polarization of macrophages by CD163/MCAM axis in glioblastoma.

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MCAM is a novel metastasis marker and regulates spreading, apoptosis and invasion of ovarian cancer cells.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Wu Z,Wu Z,Li J,Yang X,Wang Y,Yu Y,Ye J,Xu C,Qin W,Zhang Z

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Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

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Bioinformatics

Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; l-gicerin; l-gicerin protein; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen

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Gene Aliases: 1-gicerin; AV025631; CD146; CD149; MCAM; MUC18; s-endo; s-gicerin

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UniProt ID: (Human) P43121, (Rat) Q9EPF2, (Mouse) Q8R2Y2

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Entrez Gene ID: (Human) 4162, (Rat) 78967, (Mouse) 84004

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Function(s)

molecular_function

Process(es)

angiogenesis glomerular filtration cell adhesion positive regulation of cell migration vascular wound healing anatomical structure morphogenesis heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD146/MCAM Polyclonal Antibody

CD146/MCAM Antibody (17564-1-AP) in ICC/IF

Product Details

17564-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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