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Proteintech
CD146/MCAM Monoclonal Antibody (4D8A9)
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Product Details
66153-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4D8A9
Immunogen
CD146/MCAM Fusion Protein Ag11855 (26-350 aa encoded by BC056418)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
Immunogen sequence: GEAEQPAPE LVEVEVGSTA LLKCGLSQSQ GNLSHVDWFS VHKEKRTLIF RVRQGQGQSE PGEYEQRLSL QDRGATLALT QVTPQDERIF LCQGKRPRSQ EYRIQLRVYK APEEPNIQVN PLGIPVNSKE PEEVATCVGR NGYPIPQVIW YKNGRPLKEE KNRVHIQSSQ TVESSGLYTL QSILKAQLVK EDKDAQFYCE LNYRLPSGNH MKESREVTVP VFYPTEKVWL EVEPVGMLKE GDRVEIRCLA DGNPPPHFSI SKQNPSTREA EEETTNDNGV LVLEPARKEH SGRYECQGLD LDTMISLLSE PQELLVNYVS DVRVSP (26-350 aa encoded by BC056418)
Target Information
CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is heavily glycosylated, with more than 50% of its mass derived from carbohydrates. CD146 is primarily expressed on endothelial cells at cell-cell junctions, playing a crucial role in cell adhesion and the cohesion of the endothelial monolayer in vascular tissue. In addition to endothelial cells, CD146 is expressed on bone marrow fibroblasts, mesenchymal stromal cells, endometrial stromal cells, and some tumors, particularly melanoma. It has also been observed on a subset of circulating T cells and activated lymphocytes, but not on circulating endothelial cells, as indicated by the absence of other endothelial markers such as CD31 and CD51/61. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule. It is involved in the recruitment of Fyn kinase and the subsequent tyrosine phosphorylation of intracellular proteins, which are important for actin cytoskeleton assembly. This signaling role includes triggering tyrosine phosphorylation of FYN and PTK2 and causing a transient increase in intracellular calcium concentration. In the context of cancer, CD146 expression may facilitate the interaction of melanoma cells with the vascular system, enhancing hematogenous tumor spread. Additionally, CD146 could serve as an adhesion molecule active in neural crest cells during embryonic development, highlighting its diverse roles in both normal physiological processes and disease states.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
Gene Aliases: CD146; MCAM; MUC18
UniProt ID: (Human) P43121
Entrez Gene ID: (Human) 4162
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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