Invitrogen

CD15 Monoclonal Antibody (HI98), FITC, eBioscience™

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CD15 Antibody (11-0159-42) in Flow

Adrenergic signaling regulates CD11b expression in myeloid cells and controls migration into the liver after alphaGC treatment (A-E) C57BL/6J WT and Adra2ac -/- mice were treated with alphaGC (2mug/animal), and liver leukocytes were analyzed by flow cytometry after 3 h. Graphs represent the total number of liver leukocytes (CD45 +) (A), neutrophils (CD11b + Ly6G +) (B) and CD11b + F4/80 + cells (C), as well as the CD11b MFI in neutrophils (D) and CD11b + F4/80 + cells (E). (F-J) C57BL6/J WT animals were pre-treated with Yoh (5 mg/kg) or Ph... View More

CD15 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

CD15 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD15 was observed in THP-1 in comparison to low to negative in Ramos using CD15 Monoclonal Antibody (HI98), FITC, eBioscience™ (Product # 11-0159-42). {RE}

Product Details

11-0159-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgM, kappa

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI98

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11040984

Product Specific Information

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Increase of leucocyte-derived extracellular traps (ETs) in semen samples from human acute epididymitis patients-a pilot study.

Journal of assisted reproduction and genetics

Zambrano F,Schulz M,Pilatz A,Wagenlehner F,Schuppe HC,Conejeros I,Uribe P,Taubert A,Sánchez R,Hermosilla C

11-0159-42 was used in Immunocytochemistry to demonstrate the presence of neutrophilic extracellular traps used in ETosis during acute epididymitis.

Tue Sep 01 00:00:00 UTC 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 UTC 2019

A high-risk luminal A dominant breast cancer subtype with increased mobility.

Breast cancer research and treatment

Guo L,Chen G,Zhang W,Zhou L,Xiao T,Di X,Wang Y,Feng L,Zhang K

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Flow Cytometry

Sat Jun 01 00:00:00 UTC 2019

Label-free optical hemogram of granulocytes enhanced by artificial neural networks.

Optics express

Gupta RK,Chen M,Malcolm GPA,Hempler N,Dholakia K,Powis SJ

11-0159-42 was used in Flow cytometry/Cell sorting to demonstrate a comparison between artificial neural networks and principal component analysis to classify the key granulocyte cell lineages of neutrophils and eosinophils.

Mon May 13 00:00:00 UTC 2019

Multiomics Analyses of HNF4α Protein Domain Function during Human Pluripotent Stem Cell Differentiation.

iScience

Wang Y,Tatham MH,Schmidt-Heck W,Swann C,Singh-Dolt K,Meseguer-Ripolles J,Lucendo-Villarin B,Kunath T,Rudd TR,Smith AJH,Hengstler JG,Godoy P,Hay RT,Hay DC

11-0159-42 was used in Flow Cytometry to demonstrate that nuclear HNF4α is essential for hepatic progenitor specification, and the introduction of point mutations in HNF4α's Small Ubiquitin-like Modifier consensus motif leads to disrupted hepatocyte differentiation.

Fri Jun 28 00:00:00 UTC 2019

Stimulation of medulloblastoma stem cells differentiation by a peptidomimetic targeting neuropilin-1.

Oncotarget

Gong C,Valduga J,Chateau A,Richard M,Pellegrini-Moïse N,Barberi-Heyob M,Chastagner P,Boura C

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Western Blot

Fri Mar 16 00:00:00 UTC 2018

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

11-0159 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 UTC 2017

A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model.

PloS one

Yuan X,Li Z,Baines AC,Gavriilaki E,Ye Z,Wen Z,Braunstein EM,Biesecker LG,Cheng L,Dong X,Brodsky RA

11-0159 was used in Flow cytometry/Cell sorting to provide direct evidence for a critical role of glycosylphosphatidylinositol anchor proteins in early neurodevelopment.

Wed Sep 06 00:00:00 UTC 2017

Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.

The Journal of clinical investigation

Ding L,Hayes MM,Photenhauer A,Eaton KA,Li Q,Ocadiz-Ruiz R,Merchant JL

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Immunofluorescence

Mon Aug 01 00:00:00 UTC 2016

Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.

The Journal of clinical investigation

Ding L,Hayes MM,Photenhauer A,Eaton KA,Li Q,Ocadiz-Ruiz R,Merchant JL

Published figure using CD15 monoclonal antibody (Product # 11-0159-42) in Immunofluorescence

Mon Aug 01 00:00:00 UTC 2016

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

11-0159 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 UTC 2016

TNFα promotes CAR-dependent migration of leukocytes across epithelial monolayers.

Scientific reports

Morton PE,Hicks A,Ortiz-Zapater E,Raghavan S,Pike R,Noble A,Woodfin A,Jenkins G,Rayner E,Santis G,Parsons M

11-0159 was used in Flow cytometry/Cell sorting to demonstrate that trans-epithelial migration of monocyte-derived THP-1 cells is regulated by phosphorylation of the Coxsackie and Adenovirus receptor.

Thu May 19 00:00:00 UTC 2016

Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis.

Journal of immunology (Baltimore, Md. : 1950)

Bilwani FA,Knight KL

11-0159 was used in Flow cytometry/Cell sorting to investigate the factors contributing to an observed age-related decline in B lymphopoiesis.

Thu Nov 01 00:00:00 UTC 2012

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

11-0159 was used in Flow cytometry/Cell sorting to develop a humanised mouse model that recapitulates many of the classic findings in patients with sepsis.

Sat Aug 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1

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UniProt ID: (Human) P22083

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Entrez Gene ID: (Human) 2526

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Function(s)

fucosyltransferase activity alpha-(1->3)-fucosyltransferase activity

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD15 Monoclonal Antibody (HI98), FITC, eBioscience™

CD15 Antibody (11-0159-42) in Flow

Product Details

11-0159-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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