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Invitrogen
CD16 Polyclonal Antibody
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CD16 Antibody (PA5-47230) in ICC/IF
Immunocytochemistry analysis of CD16 in immersion fixed mouse splenocytes. Samples were incubated in CD16 polyclonal antibody (Product # PA5-47230) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surface.
Product Details
PA5-47230
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIII Ala31-Thr215
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609614
Product Specific Information
In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma RIIIB and rhCD32 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD16-2; CD16a; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc gamma receptor III; Fc gammaRIV; Fc receptor-like 3; Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-RIIb; Fc-gamma-RIIc; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; IgG Fc receptor III-A; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; RP11-5K23.1
Gene Aliases: 4833442P21Rik; CD16; CD16-2; FcgammaRIV; Fcgr3a; Fcgr4; FcgRIV; Fcrl3
UniProt ID: (Mouse) A0A0B4J1G0
Entrez Gene ID: (Mouse) 14131, (Mouse) 246256
antibody-dependent cellular cytotoxicity
positive regulation of type IIa hypersensitivity
positive regulation of type III hypersensitivity
positive regulation of type I hypersensitivity
serotonin secretion
phagocytosis, recognition
phagocytosis, engulfment
cell surface receptor signaling pathway
neutrophil chemotaxis
Fc-gamma receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
mast cell activation
positive regulation of phagocytosis
regulation of immune response
NK T cell proliferation
regulation of sensory perception of pain
cellular response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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