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          • Primary Antibodies ›
          • CD16 Antibodies

          Invitrogen

          CD16 Monoclonal Antibody (3G8)

          35 References
          View all (167) CD16 antibodies

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          Cite CD16 Monoclonal Antibody (3G8)

          • Antibody Testing Data (4)
          CD16 Antibody in Flow Cytometry (Flow)
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          CD16 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          CD16 Antibody (MA1-10112) in Flow

          Separation of human CD16 positive lymphocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD16 (3G8) purified Monoclonal antibody (Product # MA1-10112). Concentration in sample 2 µg/mL, GAM APC. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD16 Antibody in Flow Cytometry (Flow)
          CD16 Antibody in Flow Cytometry (Flow)
          CD16 Antibody in Flow Cytometry (Flow)
          CD16 Antibody in Flow Cytometry (Flow)

          Product Details

          MA1-10112

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Flow Cytometry (Flow)

          6 µg/mL
          View 20 publications 20 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 13 publications 13 publications
          Product Specifications

          Species Reactivity

          Human, Non-human primate

          Published species

          Human, Rhesus monkey

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3G8

          Immunogen

          Human neutrophils
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 647 
          • Alexa Fluor 700 
          • APC 
          • APC-Cyanine7
          • Biotin 
          • DyLight 594
          • FITC
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PerCP 
          • PerCP-Cyanine5.5
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          15mM sodium azide

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11155197

          Product Specific Information

          This antibody detects both 158V and 158F allotype on natural NK cells.

          Immunohistochemistry (Frozen): acetone fixation.

          Target Information

          CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

          View more View less

          Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

          View more View less

          UniProt ID: (Human) P08637, (Human) O75015

          View more View less

          Entrez Gene ID: (Human) 2214, (Human) 2215

          View more View less

          Function(s)
          IgG binding
          Process(es)
          positive regulation of defense response to virus by host immune response mitophagy in response to mitochondrial depolarization xenophagy Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response
          It has to be done as per old AB suggested Products section.
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