Invitrogen

CD16 Monoclonal Antibody (3G8), PE

3 Published Figures

8 References

View all (167) CD16 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

FIGURE: 1 / 3

CD16 Antibody (MHCD1604) in Flow

The Journal of biological chemistry 2015 - Published figure using CD16 monoclonal antibody (Product # MHCD1604) in Flow Cytometry

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Product Details

MHCD1604

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3G8

Immunogen

Human CD16

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10373687

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates.

mAbs

Kwiatkowski A,Co C,Kameoka S,Zhang A,Coughlin J,Cameron T,Chiao E,Bergelson S,Schmid Mason C

MHCD1604 was used in Flow cytometry/Cell sorting to suggest that afucosylated N-glycans are not critical for the in vitro phagocytic activity of a recombinant antibody whose target is aggregated Aβ and uses Fc effector function as part of its MOA.

Mon Jul 05 00:00:00 UTC 2021

Pulmonary immune responses to Mycobacterium tuberculosis in exposed individuals.

PloS one

Herzmann C,Ernst M,Lange C,Stenger S,Kaufmann SHE,Reiling N,Schaberg T,van der Merwe L,Maertzdorf J

MHCD1604 was used in Flow cytometry/Cell sorting to investigate the correlation between interferon-(IFN)-γ release assays and pulmonary immune responses in tuberculosis contacts in Germany.

Mon Dec 04 00:00:00 UTC 2017

Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.

The Journal of biological chemistry

Chauhan AK,Chen C,Moore TL,DiPaolo RJ

Published figure using CD16 monoclonal antibody (Product # MHCD1604) in Flow Cytometry

Fri Feb 20 00:00:00 UTC 2015

Induced expression of FcγRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-γhigh subset.

The Journal of biological chemistry

Chauhan AK,Chen C,Moore TL,DiPaolo RJ

Published figure using CD16 monoclonal antibody (Product # MHCD1604) in Flow Cytometry

Fri Feb 20 00:00:00 UTC 2015

Biochemical and pharmacological characterization of AZD1981, an orally available selective DP2 antagonist in clinical development for asthma.

British journal of pharmacology

Schmidt JA,Bell FM,Akam E,Marshall C,Dainty IA,Heinemann A,Dougall IG,Bonnert RV,Sargent CA

MHCD1604-4 was used in flow cytometry to identify and characterize a novel DP2 receptor antagonist.

Mon Apr 01 00:00:00 UTC 2013

Increased frequencies of pulmonary regulatory T-cells in latent Mycobacterium tuberculosis infection.

The European respiratory journal

Herzmann C,Ernst M,Ehlers S,Stenger S,Maertzdorf J,Sotgiu G,Lange C

MHCD1604 was used in Flow cytometry/Cell sorting to study how latent infection with M. tuberculosis, as defined by a positive M. tuberculosis-specific IGRA response on cells from the peripheral blood, is characterised by an increased frequency of Treg cells in the BAL.

Sat Dec 01 00:00:00 UTC 2012

Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.

Nature protocols

Choi KD,Vodyanik M,Slukvin II

MHCD1604 was used in Flow cytometry/Cell sorting to study how differentiation of myeloid progenitors into mature myeloid cells requires an additional 5-19 d of culture with cytokines, depending on the cell type.

Tue Mar 01 00:00:00 UTC 2011

Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors.

Blood

McIlroy D,Troadec C,Grassi F,Samri A,Barrou B,Autran B,Debré P,Feuillard J,Hosmalin A

MHCD1604 was used in flow cytometry to characterize human splenic dendritic cells.

Fri Jun 01 00:00:00 UTC 2001

Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.

Infection and immunity

Gopinath R,Hanna LE,Kumaraswami V,Perumal V,Kavitha V,Vijayasekaran V,Nutman TB

MHCD1604 was used in flow cytometry to study perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.

Sat Jan 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32; CD32 receptor 2; CDw32; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

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Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

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UniProt ID: (Human) P08637, (Human) O75015

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Entrez Gene ID: (Human) 2214, (Human) 2215

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Function(s)

IgG binding

Process(es)

positive regulation of defense response to virus by host immune response mitophagy in response to mitochondrial depolarization xenophagy Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response

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CD16 Monoclonal Antibody (3G8), PE

CD16 Antibody (MHCD1604) in Flow

Product Details

MHCD1604

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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