Invitrogen

CD161 Monoclonal Antibody (HP-3G10), PE, eBioscience™

FIGURE: 1 / 1

CD161 Antibody (12-1619-42) in Flow

Staining of Th17-polarized normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD161 PE (right). Total viable cells were used for analysis.

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Product Details

12-1619-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HP-3G10

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1272085

Product Specific Information

Description: This HP-3G10 monoclonal antibody reacts with CD161 (also known as NKR-P1A), a member of the C-type lectin superfamily. The human homologue of NK1.1 in C57BL/6 mice, CD161 is expressed on natural killer cells and is upregulated in response to IL-12. CD161 can also be detected on various T cell subsets, including memory/effector CD4+ T cells, CD8+ T cells, gamma delta TCR T cells, and a subset of CD3+ thymocytes. Finally, CD161 expression has been demonstrated on human Th17 CD4+ T cells. The function of this receptor is unclear although studies suggest a possible stimulatory role. Nevertheless, Lectin-like transcript-1 (LLT1), which is also known as osteoclast inhibitory lectin, has been identified as the ligand for CD161.

Applications Reported: This HP-3G10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-3G10 antibody has been pre-titrated and tested by flow cytometric analysis on CD4+ human peripheral T cells cultured under Th17 polarizing conditions. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.

Frontiers in immunology

Creyns B,Jacobs I,Verstockt B,Cremer J,Ballet V,Vandecasteele R,Vanuytsel T,Ferrante M,Vermeire S,Van Assche G,Ceuppens JL,Breynaert C

Published figure using CD161 monoclonal antibody (Product # 12-1619-42) in Flow Cytometry

Mon Apr 12 00:00:00 UTC 2021

Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.

Nature immunology

Cella M,Gamini R,Sécca C,Collins PL,Zhao S,Peng V,Robinette ML,Schettini J,Zaitsev K,Gordon W,Bando JK,Yomogida K,Cortez V,Fronick C,Fulton R,Lin LL,Gilfillan S,Flavell RA,Shan L,Artyomov MN,Bowman M,Oltz EM,Jelinsky SA,Colonna M

12-1619 was used in Flow cytometry/Cell sorting to demonstrate that innate lymphoid cells (ILCs) undergo conversion from ILC3s into ILC1-like cells in human tissues in vivo, requiring tissue factors and Aiolos in the process.

Thu Aug 01 00:00:00 UTC 2019

A phenotypically and functionally distinct human T_H2 cell subpopulation is associated with allergic disorders.

Science translational medicine

Wambre E,Bajzik V,DeLong JH,O'Brien K,Nguyen QA,Speake C,Gersuk VH,DeBerg HA,Whalen E,Ni C,Farrington M,Jeong D,Robinson D,Linsley PS,Vickery BP,Kwok WW

12-1619 was used in Flow cytometry/Cell sorting to identify a human TH2 cell signature in allergic diseases that could be used for response-monitoring and designing appropriate immunomodulatory strategies.

Wed Aug 02 00:00:00 UTC 2017

The loss of CCR6⁺ and CD161⁺ CD4⁺ T-cell homeostasis contributes to disease progression in SIV-infected rhesus macaques.

Mucosal immunology

McGary CS,Alvarez X,Harrington S,Cervasi B,Ryan ES,Iriele RI,Paganini S,Harper JL,Easley K,Silvestri G,Ansari AA,Lichterfeld M,Micci L,Paiardini M

12-1619 was used in Flow cytometry/Cell sorting to emphasize the importance of maintaining CCR6(+) and CD161(+) CD4(+) T-cell homeostasis, particularly in the mucosa, to prevent disease progression during pathogenic HIV/SIV infection.

Sat Jul 01 00:00:00 UTC 2017

Stability of human rapamycin-expanded CD4+CD25+ T regulatory cells.

Haematologica

Tresoldi E,Dell'Albani I,Stabilini A,Jofra T,Valle A,Gagliani N,Bondanza A,Roncarolo MG,Battaglia M

12-1619 was used in Flow cytometry/Cell sorting to demonstrate that rapamycin secures ex vivo-expanded human T-regulatory cells and provides justification for their clinical use in cell therapy-based trials.

Thu Sep 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: C-type lectin domain family 5 member B; CD161; HNKR-P1a; Killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; MGC138614; natural killer cell receptor; Natural killer cell surface protein P1A; NKR-P1A

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Gene Aliases: CD161; CLEC5B; hNKR-P1A; KLRB1; NKR; NKR-P1; NKR-P1A; NKRP1A

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UniProt ID: (Human) Q12918

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Entrez Gene ID: (Human) 3820

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Function(s)

transmembrane signaling receptor activity carbohydrate binding

Process(es)

cell surface receptor signaling pathway regulation of immune response

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CD161 Monoclonal Antibody (HP-3G10), PE, eBioscience™

CD161 Antibody (12-1619-42) in Flow

Product Details

12-1619-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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