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Invitrogen
CD161 Monoclonal Antibody (HP-3G10), Alexa Fluor™ 700
FIGURE: 1 / 3
CD161 Antibody (MA5-38743) in Flow
Flow cytometry of CD161 in human CD161 positive CD4 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Samples were incubated with CD161 monoclonal antibody (Product # MA5-38743) followed by Alexa Fluor 700 at a dilution of 4 µL reagent/100 µL of peripheral whole blood.
Product Details
MA5-38743
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HP-3G10
Immunogen
human NK cells
Conjugate
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898655
Product Specific Information
This antibody recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
Target Information
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-type lectin domain family 5 member B; CD161; HNKR-P1a; Killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; MGC138614; natural killer cell receptor; Natural killer cell surface protein P1A; NKR-P1A
Gene Aliases: CD161; CLEC5B; hNKR-P1A; KLRB1; NKR; NKR-P1; NKR-P1A; NKRP1A
UniProt ID: (Human) Q12918
Entrez Gene ID: (Human) 3820
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