Invitrogen

CD166 (ALCAM) Monoclonal Antibody (3A6), PE, eBioscience™

2 Published Figures

6 References

View all (62) CD166 (ALCAM) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD166 (ALCAM) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD166 (ALCAM) Antibody (12-1668-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD166 (ALCAM) PE (purple histogram). Cells in the monocyte gate were used for analysis.

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Product Details

12-1668-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3A6

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572587

Product Specific Information

Description: This 3A6 monoclonal antibody reacts with human CD166, which is also known as activated leukocyte cell adhesion molecule (ALCAM). CD166 is an immunoglobulin superfamily cell adhesion molecule that is broadly expressed on developing and adult hematopoietic (e.g., activated T and B cells, monocytes, and dendritic cells) and non-hematopoietic (e.g., neural, endothelial, and epithelial) tissues. CD166 has also been detected in melanoma, colorectal carcinoma, as well as cancers of the breast, prostate, and bladder. CD166 mediates cell-cell adhesion by forming low-affinity homophilic interactions (ALCAM-ALCAM) or high-affinity heterophilic interactions with its ligand CD6 (ALCAM-CD6). CD166 plays a role in development, inflammation, and neutrophil migration. Moreover, its expression can be used as a prognostic marker for several cancers and has been reported to be a cancer stem cell marker.

The 3A6 antibody has been reported to induce the internalization of CD166 and its recycling back to the cell surface.

Applications Reported: This 3A6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Mesenchymal stem cells negatively regulate CD4⁺ T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.

Molecular medicine reports

Gong B,Zheng L,Lu Z,Huang J,Pu J,Pan S,Zhang M,Liu J,Tang J

12-1668 was used in Flow cytometry/Cell sorting to indicate that the expression levels of miRNA‚125b‚5p and miRNA‚155 in TCR‚activated CD4+ T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment.

Fri Jan 01 00:00:00 UTC 2021

In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium.

Stem cells international

Han B,Zhou L,Guan Q,da Roza G,Wang H,Du C

12-1668 was used in Flow cytometry/Cell sorting to show that upon expansion in a human protein medium, pMSCs shows a differential MSC marker expression profile from those of bone marrow or adipose tissue-derived MSCs and could maintain the multipotency.

Wed Sep 30 00:00:00 UTC 2020

Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer.

Cell reports

Barros-Silva JD,Linn DE,Steiner I,Guo G,Ali A,Pakula H,Ashton G,Peset I,Brown M,Clarke NW,Bronson RT,Yuan GC,Orkin SH,Li Z,Baena E

12-1668 was used in Flow cytometry/Cell sorting to analyse the molecular heterogeneity in basal and luminal prostate compartments.

Tue Dec 18 00:00:00 UTC 2018

Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.

Cell death & disease

Ilmer M,Mazurek N,Byrd JC,Ramirez K,Hafley M,Alt E,Vykoukal J,Bresalier RS

Published figure using CD166 (ALCAM) monoclonal antibody (Product # 12-1668-42) in Flow Cytometry

Thu Aug 11 00:00:00 UTC 2016

Enrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19.

Stem cells and development

Bauderlique-Le Roy H,Vennin C,Brocqueville G,Spruyt N,Adriaenssens E,Bourette RP

Published figure using CD166 (ALCAM) monoclonal antibody (Product # 12-1668-42) in Flow Cytometry

Fri May 15 00:00:00 UTC 2015

A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.

Arthritis & rheumatology (Hoboken, N.J.)

Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

12-1668 was used in Flow cytometry/Cell sorting to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients.

Fri Aug 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Activated leukocyte cell adhesion molecule; CD166; CD166 antigen; FLJ38514; MGC71733

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Gene Aliases: ALCAM; CD166; MEMD

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UniProt ID: (Human) Q13740

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Entrez Gene ID: (Human) 214

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Function(s)

receptor binding protein binding

Process(es)

adaptive immune response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules signal transduction motor neuron axon guidance retinal ganglion cell axon guidance axon extension involved in axon guidance neuron projection extension

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD166 (ALCAM) Monoclonal Antibody (3A6), PE, eBioscience™

CD166 (ALCAM) Antibody (12-1668-42) in Flow

Product Details

12-1668-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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