Invitrogen

CD166 (ALCAM) Monoclonal Antibody (eBioALC48), PE, eBioscience™

5 Published Figures

16 References

View all (62) CD166 (ALCAM) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

CD166 (ALCAM) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD166 (ALCAM) Antibody (12-1661-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.125 µg of Anti-Mouse CD166 (ALCAM) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-1661-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioALC48

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_823125

Product Specific Information

Description: Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. ALCAM is a type I transmembrane glycoprotein, possessing five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion, as well as heterotypic adhesion with its natural ligand, CD6. A soluble isoform of ALCAM has been shown to inhibit homophilic adhesive interactions. ALCAM is expressed on a variety of cell types, including activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. ALCAM expression has also been used as a marker for the isolation of pluripotent stem cells.

Applications Reported: This eBioALC-48 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioALC-48 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration.

Stem cell research & therapy

Dushime H,Moreno SG,Linard C,Adrait A,Couté Y,Peltzer J,Messiaen S,Torres C,Bensemmane L,Lewandowski D,Romeo PH,Petit V,Gault N

12-1661-82 was used in Flow cytometry/Cell sorting to indicate that a single injection of a small quantity of WJ-Muse may be a new and easy therapeutic strategy for treating lethal GIS.

Fri Aug 11 00:00:00 UTC 2023

NFATc1 marks articular cartilage progenitors and negatively determines articular chondrocyte differentiation.

eLife

Zhang F,Wang Y,Zhao Y,Wang M,Zhou B,Zhou B,Ge X

Published figure using CD166 (ALCAM) monoclonal antibody (Product # 12-1661-82) in Flow Cytometry

Wed Feb 15 00:00:00 UTC 2023

Suppression of Experimental Autoimmune Encephalomyelitis by ILT3.Fc.

Journal of immunology (Baltimore, Md. : 1950)

Xu Z,Lin CC,Ho S,Vlad G,Suciu-Foca N

12-1661-82 was used in Flow Cytometry to explore the role of recombinant human ILT3.Fc protein in the pathophysiology of multiple sclerosis.

Mon Feb 01 00:00:00 UTC 2021

Murine Oncostatin M Has Opposing Effects on the Proliferation of OP9 Bone Marrow Stromal Cells and NIH/3T3 Fibroblasts Signaling through the OSMR.

International journal of molecular sciences

Jakob L,Müller TA,Rassner M,Kleinfelder H,Veratti P,Mitschke J,Miething C,Oostendorp RAJ,Pfeifer D,Waterhouse M,Duyster J

12-1661-82 was used in Flow cytometry to investigate the effect of murine Oncostatin M on proliferation in stromal and fibroblast cell lines.

Thu Oct 28 00:00:00 UTC 2021

The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma.

EMBO molecular medicine

Lin S,Huang C,Sun J,Bollt O,Wang X,Martine E,Kang J,Taylor MD,Fang B,Singh PK,Koomen J,Hao J,Yang S

12-1661 was used in Flow cytometry/Cell sorting to identify the role of mitochondrial deoxynucleotide triphosphate metabolism in lunge cancer tumor growth and progression.

Sun Dec 01 00:00:00 UTC 2019

The mitochondrial deoxyguanosine kinase is required for cancer cell stemness in lung adenocarcinoma.

EMBO molecular medicine

Lin S,Huang C,Sun J,Bollt O,Wang X,Martine E,Kang J,Taylor MD,Fang B,Singh PK,Koomen J,Hao J,Yang S

12-1661 was used in Flow cytometry/Cell sorting to identify the role of mitochondrial deoxynucleotide triphosphate metabolism in lunge cancer tumor growth and progression.

Sun Dec 01 00:00:00 UTC 2019

Generation of mesothelial progenitor-like cells from mouse-induced pluripotent stem cells.

FEBS letters

Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

12-1661-82 was used in Flow Cytometry to defined factors, as well as purification using MPC-specific cell surface antigens.

Fri Feb 01 00:00:00 UTC 2019

Isolation of a unique hepatic stellate cell population expressing integrin α8 from embryonic mouse livers.

Developmental dynamics : an official publication of the American Association of Anatomists

Ogawa T,Li Y,Lua I,Hartner A,Asahina K

12-1661 was used in Flow cytometry/Cell sorting to suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development.

Fri Jun 01 00:00:00 UTC 2018

In vivo hematopoietic Myc activation directs a transcriptional signature in endothelial cells within the bone marrow microenvironment.

Oncotarget

Franke K,Vilne B,Prazeres da Costa O,Rudelius M,Peschel C,Oostendorp RA,Keller U

12-1661 was used in Flow cytometry/Cell sorting to study the effect of Myc activation on the frequency of different hematopoietic stem/ progenitor cell subsets.

Tue Sep 08 00:00:00 UTC 2015

Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.

PloS one

Wiencierz AM,Kernbach M,Ecklebe J,Monnerat G,Tomiuk S,Raulf A,Christalla P,Malan D,Hesse M,Bosio A,Fleischmann BK,Eckardt D

12-1661 was used in Flow cytometry/Cell sorting to introduce a non-genetic, antibody-based approach to specifically isolate highly pure and viable atrial and ventricular cardiomyocytes from mouse hearts of various developmental stages.

Fri Jun 17 00:00:00 UTC 2016

CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche.

Blood

Chitteti BR,Kobayashi M,Cheng Y,Zhang H,Poteat BA,Broxmeyer HE,Pelus LM,Hanenberg H,Zollman A,Kamocka MM,Carlesso N,Cardoso AA,Kacena MA,Srour EF

12-1661 was used in Flow cytometry/Cell sorting to demonstrate that CD166 is a functional haematopoietic stem cell marker that identifies both murine and human long-term repopulating cells.

Thu Jul 24 00:00:00 UTC 2014

Cellular heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population.

Cell reports

DeWard AD,Cramer J,Lagasse E

Published figure using CD166 (ALCAM) monoclonal antibody (Product # 12-1661-82) in Flow Cytometry

Thu Oct 23 00:00:00 UTC 2014

Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.

Nature protocols

Smith AJ,Lewis FC,Aquila I,Waring CD,Nocera A,Agosti V,Nadal-Ginard B,Torella D,Ellison GM

12-1661-82 was used in Flow Cytometry to isolate endogenous c-Kit (also known as CD117)-positive (c-Kit(+)), CD45-negative (CD45(-)) cardiac stem cells (eCSCs) from whole adult mouse and rat hearts.

Tue Jul 01 00:00:00 UTC 2014

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

12-1661 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 UTC 2014

Bone marrow-derived cell therapy stimulates endogenous cardiomyocyte progenitors and promotes cardiac repair.

Cell stem cell

Loffredo FS,Steinhauser ML,Gannon J,Lee RT

12-1661 was used in Flow cytometry/Cell sorting to stimulate endogenous cardiomyocyte progenitors and promote cardiac repair as part of bone marrow-derived cell therapy.

Fri Apr 08 00:00:00 UTC 2011

Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability.

The Journal of biological chemistry

Xu D,Fuster MM,Lawrence R,Esko JD

12-1661 was used in Flow cytometry/Cell sorting to demonstrate how VEGF-induced vascular hyper-permeability depends on endothelial heparan sulfate.

Fri Jan 07 00:00:00 UTC 2011

Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development.

Hepatology (Baltimore, Md.)

Asahina K,Tsai SY,Li P,Ishii M,Maxson RE,Sucov HM,Tsukamoto H

Published figure using CD166 (ALCAM) monoclonal antibody (Product # 12-1661-82) in Flow Cytometry

Sun Mar 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Activated leukocyte cell adhesion molecule; BEN; CD166; CD166 antigen; FLJ38514; MGC71733; Protein DM-GRASP

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Gene Aliases: AI853494; Alcam; BEN; CD166; DM-GRASP; MuSC; SC1

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UniProt ID: (Mouse) Q61490

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Entrez Gene ID: (Mouse) 11658

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Function(s)

protein binding

Process(es)

cell adhesion axon guidance motor neuron axon guidance

Performance Guarantee

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CD166 (ALCAM) Monoclonal Antibody (eBioALC48), PE, eBioscience™

CD166 (ALCAM) Antibody (12-1661-82) in Flow

Product Details

12-1661-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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