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Invitrogen
CD166 (ALCAM) Monoclonal Antibody (eBioALC48), eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD166 (ALCAM) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 17
CD166 (ALCAM) Antibody (14-1661-82) in Flow
Staining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD166 (ALCAM) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD166 (ALCAM) Antibody (14-1661-82) in Flow")
Product Details
14-1661-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioALC48
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_823127
Product Specific Information
Description: Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a 105 kD cell-surface adhesion molecule belonging to the Ig superfamily of receptors. ALCAM is a type I transmembrane glycoprotein, possessing five extracellular Ig domains, a transmembrane domain, and a short cytoplasmic tail. ALCAM is involved in homotypic adhesion, as well as heterotypic adhesion with its natural ligand, CD6. A soluble isoform of ALCAM has been shown to inhibit homophilic adhesive interactions. ALCAM is expressed on a variety of cell types, including activated T and B cells, monocyte-derived dendritic cells, and thymic epithelial cells. ALCAM expression has also been used as a marker for the isolation of pluripotent stem cells.
Applications Reported: This eBioALC-48 antibody has been reported for use in flow cytometric analysis and immunohistochemistry on paraffin-embedded tissue sections.
Applications Tested: The eBioALC-48 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activated leukocyte cell adhesion molecule; BEN; CD166; CD166 antigen; FLJ38514; MGC71733; Protein DM-GRASP
Gene Aliases: AI853494; Alcam; BEN; CD166; DM-GRASP; MuSC; SC1
UniProt ID: (Mouse) Q61490
Entrez Gene ID: (Mouse) 11658
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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