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Invitrogen
CD166 Polyclonal Antibody
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FIGURE: 1 / 7
CD166 Antibody (PA5-47083) in ICC/IF
Immunocytochemical analysis of ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47083) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
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.jpg?time=20250104?time=20250104 "CD166 Antibody (PA5-47083) in IHC (F)")
Product Details
PA5-47083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 Trp28-Lys527
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607383
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ALCAM is observed, and less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activated leukocyte cell adhesion molecule; BEN; CD166; CD166 antigen; FLJ38514; HB2; KG-CAM; MGC71733; Protein DM-GRASP; Protein MEMD; SB-10 antigen
Gene Aliases: AI853494; ALCAM; BEN; CD166; DM-GRASP; MEMD; MuSC; SC1
UniProt ID: (Human) Q13740, (Rat) O35112, (Mouse) Q61490
Entrez Gene ID: (Human) 214, (Dog) 478550, (Rat) 79559, (Mouse) 11658
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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